Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SG34_RS03730 Genome accession   NZ_CP059733
Coordinates   839068..840768 (-) Length   566 a.a.
NCBI ID   WP_053046400.1    Uniprot ID   A0AAF0CA16
Organism   Thalassomonas viridans strain XOM25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 834068..845768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SG34_RS03700 (SG34_003675) mutT 834262..834669 (+) 408 WP_044842369.1 8-oxo-dGTP diphosphatase MutT -
  SG34_RS03705 (SG34_003680) yacG 834693..834929 (-) 237 WP_044842370.1 DNA gyrase inhibitor YacG -
  SG34_RS03710 (SG34_003685) zapD 835008..835757 (-) 750 WP_044842371.1 cell division protein ZapD -
  SG34_RS03715 (SG34_003690) coaE 835988..836599 (-) 612 WP_044842372.1 dephospho-CoA kinase -
  SG34_RS03720 (SG34_003695) pilD 836668..837552 (-) 885 WP_274038509.1 prepilin peptidase Machinery gene
  SG34_RS03725 (SG34_003700) pilC 837766..839007 (-) 1242 WP_044836840.1 type II secretion system F family protein Machinery gene
  SG34_RS03730 (SG34_003705) pilB 839068..840768 (-) 1701 WP_053046400.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SG34_RS03735 (SG34_003710) - 840947..841363 (-) 417 WP_236701185.1 pilin -
  SG34_RS03740 (SG34_003715) nadC 841747..842637 (-) 891 WP_044836842.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SG34_RS03745 (SG34_003720) - 842641..843159 (-) 519 WP_044836843.1 retropepsin-like aspartic protease family protein -
  SG34_RS03750 (SG34_003725) ampD 843198..843785 (+) 588 WP_044836844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SG34_RS03755 (SG34_003730) ampE 843839..844813 (+) 975 WP_044836845.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62590.72 Da        Isoelectric Point: 5.2291

>NTDB_id=471640 SG34_RS03730 WP_053046400.1 839068..840768(-) (pilB) [Thalassomonas viridans strain XOM25]
MKGHHQQSSLLSALSKHNLVPEELVDEISATFLQQKKPFIRYLVEDKNYNAVSIVSILSRSFGYPHIDLKNFDTSLVPDG
IRNEKLIHKHNALPLFLRGKVLFVAMSDPTNLDALEEIQFNTGFATELVLTDENSLQSCIDKVLEEDSEALGITDIDAEE
LSDIDVQEERKEDEVGEDKDDAPIVVYINKILLDAIRKGASDLHFEPYERSYRIRFRIDGILKEVAKPPVTLASRMAARL
KVMSKLDIAERRIPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDMLGYEADQKAIYMDALAQPQG
MILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLEGINQVQINNKAGLTFPSALRSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCPQCKTEEIIPHEALAKQGFTP
EQIKDIKLYKAVGCEQCTGGYRGRVGIYEVIKISQETANIIMAGGNSLDIAAQCQTEGFNNLRQSGLVKAVQGVTSLEEV
NRVTSA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=471640 SG34_RS03730 WP_053046400.1 839068..840768(-) (pilB) [Thalassomonas viridans strain XOM25]
ATGAAAGGACACCATCAGCAGTCAAGCTTACTCAGCGCCCTGTCCAAGCACAATTTAGTGCCAGAAGAACTGGTTGATGA
AATCAGCGCAACTTTTTTGCAGCAAAAAAAACCCTTTATCCGCTATCTGGTTGAAGATAAAAACTATAATGCCGTCAGCA
TTGTCAGCATCTTGTCCCGCAGCTTTGGTTACCCCCATATAGACCTAAAGAACTTTGATACTTCCTTAGTGCCCGACGGC
ATCCGCAACGAAAAACTGATCCATAAGCATAACGCTCTGCCGTTGTTTTTACGTGGCAAGGTACTGTTTGTGGCCATGTC
GGATCCCACCAATTTAGATGCCCTGGAAGAAATCCAGTTTAATACCGGGTTTGCCACCGAACTGGTATTAACGGATGAAA
ACAGCCTGCAAAGCTGTATAGACAAGGTGCTGGAAGAAGACAGCGAAGCCCTGGGGATCACAGATATAGATGCCGAAGAA
CTGTCCGATATAGATGTCCAGGAAGAGCGTAAAGAAGACGAGGTCGGCGAAGACAAAGACGACGCGCCGATTGTTGTGTA
CATCAATAAAATCTTGCTGGACGCCATCCGCAAAGGCGCGTCCGATTTGCACTTCGAGCCTTATGAAAGATCTTACCGCA
TCCGTTTCCGTATCGACGGCATCTTAAAAGAAGTAGCCAAGCCCCCGGTCACCCTGGCTTCGCGTATGGCTGCCAGGCTA
AAGGTCATGTCCAAACTGGATATTGCCGAGCGGCGTATTCCCCAGGACGGCCGGATTAAACTGGCGCTATCCAAGAAAAA
ATCTATCGATTTTCGTGTCAGTACCCTGCCCACCATGTGGGGGGAGAAAATCGTGATGCGTATCCTCGACTCTTCCAGCG
CCATGCTCGGCATCGACATGCTCGGCTATGAAGCGGATCAAAAAGCCATCTACATGGACGCCCTTGCCCAGCCGCAAGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCAGGTAAAACCGTTTCTTTATATACGGGCTTAAACATCTTGAACACCCAGGA
GCGCAATATCTCCACCGCCGAAGACCCGGTTGAAATCAACCTGGAAGGCATCAACCAGGTACAGATCAACAACAAGGCCG
GGTTAACCTTCCCCAGCGCCCTGCGCTCTTTTTTACGGCAAGACCCGGATATTGTTATGGTGGGTGAAATCCGGGATCTG
GAAACCGCCGAAATTGCCATTAAAGCGGCACAAACCGGGCACCTGGTATTGTCGACCCTGCATACCAATTCCGCGGCAGA
AACCCTGACCCGGCTGCTGAATATGGGAGTGCCTTCCTATAATGTCGCCAGCTCCGTCAGTATTATCATTGCCCAACGCC
TGGCCCGGCGCTTGTGCCCCCAATGTAAAACCGAAGAAATCATTCCCCATGAAGCCCTGGCTAAGCAGGGCTTTACCCCC
GAGCAAATAAAAGACATTAAACTATATAAAGCGGTGGGCTGCGAACAATGCACCGGGGGCTATCGGGGAAGGGTCGGCAT
TTATGAAGTTATCAAGATCAGCCAGGAAACCGCCAATATCATCATGGCGGGCGGCAACAGCCTGGATATTGCAGCCCAGT
GCCAGACCGAAGGATTTAATAACTTACGCCAGTCCGGCCTGGTCAAAGCCGTGCAGGGCGTAACCAGTTTAGAAGAAGTG
AACCGGGTAACCAGTGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.74

95.23

0.569
  pilB Acinetobacter baylyi ADP1

59.439

94.523

0.562
  pilB Legionella pneumophila strain ERS1305867

56.655

98.233

0.557
  pilB Vibrio cholerae strain A1552

55.492

93.286

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.371

96.643

0.496
  pilB Vibrio campbellii strain DS40M4

51.005

96.643

0.493
  pilF Neisseria gonorrhoeae MS11

51.119

94.7

0.484