Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H3N35_RS23960 Genome accession   NZ_CP059693
Coordinates   5546202..5547902 (+) Length   566 a.a.
NCBI ID   WP_274051360.1    Uniprot ID   -
Organism   Thalassomonas haliotis strain A5K-61T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5541202..5552902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3N35_RS23935 (H3N35_23795) ampE 5542046..5543077 (-) 1032 WP_274051352.1 beta-lactamase regulator AmpE -
  H3N35_RS23940 (H3N35_23800) ampD 5543124..5543711 (-) 588 WP_274051353.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H3N35_RS23945 (H3N35_23805) - 5543750..5544268 (+) 519 WP_274051354.1 retropepsin-like aspartic protease family protein -
  H3N35_RS23950 (H3N35_23810) nadC 5544272..5545162 (+) 891 WP_274051356.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H3N35_RS23955 (H3N35_23815) - 5545597..5546025 (+) 429 WP_274051358.1 pilin -
  H3N35_RS23960 (H3N35_23820) pilB 5546202..5547902 (+) 1701 WP_274051360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3N35_RS23965 (H3N35_23825) pilC 5547972..5549213 (+) 1242 WP_274051361.1 type II secretion system F family protein Machinery gene
  H3N35_RS23970 (H3N35_23830) pilD 5549265..5550149 (+) 885 WP_274051362.1 prepilin peptidase Machinery gene
  H3N35_RS23975 (H3N35_23835) coaE 5550225..5550836 (+) 612 WP_274051364.1 dephospho-CoA kinase -
  H3N35_RS23980 (H3N35_23840) zapD 5551067..5551816 (+) 750 WP_274051365.1 cell division protein ZapD -
  H3N35_RS23985 (H3N35_23845) yacG 5551903..5552139 (+) 237 WP_274051366.1 DNA gyrase inhibitor YacG -
  H3N35_RS23990 (H3N35_23850) mutT 5552163..5552561 (-) 399 WP_274051367.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62651.79 Da        Isoelectric Point: 5.1585

>NTDB_id=471360 H3N35_RS23960 WP_274051360.1 5546202..5547902(+) (pilB) [Thalassomonas haliotis strain A5K-61T]
MKGHHQQSSLLSALSKHNLVPEELVDEISATFLQQKKPFIRYLVEDKNYNAVSIVSILSRSFGYPHIDLKNFDTSLVPEG
IRNEKLIHKHNALPLFLRGKVLFVAMSDPTNLDALEEIQFNTGFATELVLTDENSLQTCIDKVLEEDSEALGITDIDAEE
LSEIDVQEERKEDETGEDKDDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRIDGILNEVAKPPVTLASRMAARL
KVMSKLDIAERRIPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIEMLGYEAAQKEIYLEALARPQG
MILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLEGINQVQINNKAGLTFPSALRSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCGQCKTEEVIPQEALLKQGFTP
EQVKDIKLYKAVGCEQCTGGYRGRVGIYEVIRISQETANIIMAGGNSLDIAAQCQTEGFNNLRQSGLIKAVQGVTSLEEV
NRVTSA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=471360 H3N35_RS23960 WP_274051360.1 5546202..5547902(+) (pilB) [Thalassomonas haliotis strain A5K-61T]
ATGAAAGGACACCATCAACAGTCAAGCTTACTCAGCGCCCTGTCCAAGCACAACTTAGTGCCTGAAGAACTGGTTGATGA
AATCAGCGCGACTTTTTTACAACAAAAAAAGCCGTTTATCCGCTATTTAGTTGAAGACAAAAACTACAATGCCGTCAGCA
TTGTCAGTATCTTATCCCGCAGCTTTGGCTATCCCCATATTGACTTAAAAAACTTTGATACCTCTTTGGTGCCCGAAGGT
ATCCGCAACGAAAAGCTGATCCACAAGCATAATGCCCTGCCGCTGTTTTTACGCGGAAAGGTTTTATTTGTCGCCATGTC
AGATCCCACTAATCTGGATGCCCTGGAGGAGATACAATTTAATACCGGCTTTGCAACCGAGCTGGTACTGACCGATGAAA
ACAGTCTGCAAACCTGTATAGACAAGGTACTGGAAGAAGACAGCGAAGCCCTGGGCATTACCGACATTGATGCGGAAGAA
CTTTCCGAGATAGATGTCCAGGAAGAACGCAAAGAAGACGAAACCGGTGAAGACAAAGACGACGCTCCCATTGTCGTTTA
TATCAATAAAATCTTATTGGACGCCATCAGAAAGGGGGCATCCGATTTACATTTCGAACCTTATGAGAAGTCTTACCGCA
TTCGTTTTCGTATTGACGGCATCTTAAACGAAGTGGCTAAACCCCCGGTAACTCTCGCATCCCGTATGGCGGCCCGGTTA
AAAGTCATGTCAAAACTCGATATAGCCGAGCGCCGCATCCCCCAGGATGGCCGGATCAAACTCGCCTTATCCAAGAAGAA
ATCCATCGACTTTCGTGTCAGTACCCTGCCTACCATGTGGGGTGAAAAAATCGTAATGCGTATCCTCGACTCATCCAGCG
CCATGTTGGGCATAGAGATGTTGGGATATGAAGCCGCCCAAAAAGAAATCTACCTGGAAGCACTCGCCCGGCCGCAAGGC
ATGATTCTCGTCACCGGCCCCACCGGCTCAGGCAAAACCGTTTCTTTATATACCGGGTTAAATATCTTGAATACCCAGGA
GCGCAATATCTCCACCGCAGAAGATCCGGTTGAGATCAACCTCGAAGGCATCAACCAGGTACAAATCAATAATAAAGCCG
GCTTAACCTTCCCCAGCGCCCTGCGCTCTTTTTTAAGACAAGATCCCGATATTGTTATGGTCGGTGAGATCCGGGATCTC
GAAACCGCAGAAATCGCCATTAAAGCCGCCCAAACCGGGCACTTAGTGTTATCCACCCTGCATACCAACTCTGCGGCAGA
AACCCTCACCCGGCTGCTGAATATGGGCGTACCTTCTTATAATGTTGCCAGCTCCGTTAGCATCATTATTGCCCAGCGCC
TGGCCCGGCGCTTATGCGGCCAATGCAAAACCGAAGAAGTTATTCCGCAAGAGGCCCTGCTTAAGCAAGGTTTTACCCCG
GAACAAGTAAAAGATATCAAATTATACAAGGCTGTCGGCTGCGAGCAATGCACCGGGGGTTACCGGGGCAGGGTTGGTAT
TTACGAAGTCATCAGGATCAGCCAGGAAACGGCCAACATCATTATGGCGGGAGGCAACAGCCTGGATATCGCCGCCCAAT
GTCAAACCGAAGGATTTAATAATTTACGCCAGTCAGGCCTGATCAAGGCCGTACAAGGCGTGACCAGTTTAGAAGAAGTT
AACCGGGTAACAAGCGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.066

98.763

0.564
  pilB Acinetobacter baylyi ADP1

59.439

94.523

0.562
  pilB Acinetobacter baumannii D1279779

58.879

94.523

0.557
  pilB Vibrio cholerae strain A1552

55.682

93.286

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

52.468

96.643

0.507
  pilF Neisseria gonorrhoeae MS11

51.493

94.7

0.488
  pilB Vibrio campbellii strain DS40M4

53.922

90.106

0.486