Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L91_RS00280 Genome accession   NZ_CP059571
Coordinates   49598..51271 (-) Length   557 a.a.
NCBI ID   WP_007341357.1    Uniprot ID   -
Organism   Neisseria bacilliformis strain DSM 23338     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 44598..56271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L91_RS00250 (H3L91_00250) - 45117..45725 (-) 609 WP_007341351.1 HI_0552 family protein -
  H3L91_RS00255 (H3L91_00255) - 45905..46090 (-) 186 WP_007341352.1 DNA gyrase inhibitor YacG -
  H3L91_RS00260 (H3L91_00260) zapD 46094..46855 (-) 762 WP_007341353.1 cell division protein ZapD -
  H3L91_RS00265 (H3L91_00265) coaE 46860..47471 (-) 612 WP_007341354.1 dephospho-CoA kinase -
  H3L91_RS00270 (H3L91_00270) pilD 47468..48349 (-) 882 WP_007341355.1 A24 family peptidase Machinery gene
  H3L91_RS00275 (H3L91_00275) pilG 48356..49579 (-) 1224 WP_007341356.1 type II secretion system F family protein Machinery gene
  H3L91_RS00280 (H3L91_00280) pilF 49598..51271 (-) 1674 WP_007341357.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L91_RS00285 (H3L91_00285) - 51420..53009 (-) 1590 WP_040658482.1 inorganic phosphate transporter -
  H3L91_RS00290 (H3L91_00290) - 53530..54402 (-) 873 WP_007341361.1 EamA family transporter -
  H3L91_RS00295 (H3L91_00295) - 54723..55085 (+) 363 WP_007341363.1 YbaN family protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61961.20 Da        Isoelectric Point: 5.4233

>NTDB_id=470852 H3L91_RS00280 WP_007341357.1 49598..51271(-) (pilF) [Neisseria bacilliformis strain DSM 23338]
MSVGLLRVLAQNRILSAEQVERYQKALGAGKDIIPMLFNDNVTDPKSLAELLAGIFNYPVLDLTYYPRSNIVQDILPEEQ
MQKNNCIPIFKRGGKLYLAVSDPTRIQEFQKLITGYSADLIVVRHDQLLTLTEWLAQRSTTILREMSQEQLDTAAPQALF
IDDQEAEDGPIARFIQKTLADALNAGASDIHFEFYEHTARVRFRVDGQLREVVQPPVAVRSQLASRIKVMAKLDISEKRI
PQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDASSLNIDQLGFEDFQKDLLLEAIHRPYGMVLVTGPTGSGK
TVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSNCKEPMDRPPEHALKKAGFTEEDLAKDWTMYR
AVGCDTCRGKGYKGRAGVYEVMPITDEMQRVIMEGGTEVDIQQMAYKEGMVDLRRAGLLKVMDGITSLEEVLGNTND

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=470852 H3L91_RS00280 WP_007341357.1 49598..51271(-) (pilF) [Neisseria bacilliformis strain DSM 23338]
ATGAGTGTAGGATTATTAAGGGTTTTAGCGCAAAACCGCATACTGTCCGCCGAGCAGGTCGAACGGTATCAGAAGGCATT
GGGGGCGGGAAAAGACATCATCCCCATGCTCTTTAATGATAACGTTACCGACCCGAAGTCTCTGGCCGAACTGCTGGCCG
GAATCTTCAACTACCCTGTTTTGGATCTGACCTATTACCCGCGCAGCAACATCGTGCAGGACATCCTGCCGGAAGAGCAG
ATGCAGAAAAACAACTGCATCCCGATCTTCAAGCGGGGCGGGAAACTCTATCTGGCCGTGTCCGATCCGACACGGATTCA
GGAATTCCAAAAACTGATCACCGGCTATTCCGCCGACCTGATTGTCGTGCGCCACGATCAGCTTTTGACACTGACCGAAT
GGCTGGCGCAGCGTTCCACCACTATTTTGCGCGAAATGTCGCAGGAACAGTTGGATACCGCTGCGCCGCAGGCTTTGTTC
ATCGACGATCAGGAAGCGGAAGACGGGCCGATTGCGCGGTTTATCCAAAAAACCTTGGCCGACGCTTTGAATGCCGGCGC
ATCGGATATCCATTTCGAATTTTACGAACACACCGCACGCGTCCGTTTCCGTGTGGACGGGCAATTGCGCGAAGTTGTTC
AGCCGCCTGTCGCCGTGCGCAGCCAGTTGGCTTCGCGCATCAAAGTGATGGCCAAACTCGACATCTCCGAAAAACGCATT
CCGCAGGACGGCCGCATCCAAATCGCCTTCTCCAAGCACGGCCGCCCGACAGACTTCCGTGTCAGCACACTGCCGACCCT
GTTCGGCGAAAAAGTGGTGATGCGTATTTTGAATTCCGATGCCTCCTCTCTGAATATTGATCAGCTCGGTTTTGAGGACT
TCCAAAAAGATTTGCTGCTCGAAGCTATTCACCGCCCGTACGGCATGGTTCTGGTAACCGGCCCGACCGGTTCGGGTAAA
ACAGTTTCGCTCTATACCTGCTTGAACATTCTGAATACCGAGGGTGTGAACATTTCCACGGCGGAAGACCCCGCCGAGAT
CAACCTCCCGGGCATCAATCAGGTAAACGTAAACGACAAACAGGGTCTGACCTTTGCCGCCGCGTTGAAATCCTTCCTGC
GCCAAGACCCGGACATCATCATGGTGGGTGAGATCCGCGACTTGGAAACGGCGGACATCGCCATTAAGGCGGCACAAACA
GGCCATATGGTGTTTTCCACCCTGCATACCAACAATGCGCCGGCCACTCTGTCCCGTATGCTGAACATGGGCGTTGCGCC
TTTCAATATTGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGGCGGCTTTGTTCAAACTGTAAAGAACCGA
TGGACAGACCGCCCGAACACGCGCTGAAAAAAGCCGGCTTTACCGAAGAAGATTTGGCCAAAGACTGGACGATGTACCGC
GCGGTCGGCTGCGATACCTGCCGAGGAAAAGGATACAAAGGCCGTGCCGGCGTATATGAAGTTATGCCGATTACCGACGA
AATGCAGCGCGTCATCATGGAAGGCGGCACCGAAGTGGACATTCAGCAAATGGCTTACAAAGAAGGAATGGTCGATCTGC
GCCGAGCCGGTTTGCTGAAGGTAATGGACGGAATCACCTCTTTGGAAGAAGTATTGGGCAACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-136)

Type II/IV secretion system protein (T2SSE)

(177-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

74.24

100

0.745
  pilB Acinetobacter baumannii D1279779

52.22

100

0.528
  pilB Acinetobacter baylyi ADP1

51.259

99.82

0.512
  pilB Legionella pneumophila strain ERS1305867

48.932

100

0.494
  pilB Vibrio cholerae strain A1552

47.069

100

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

45.133

100

0.458
  pilB Vibrio campbellii strain DS40M4

43.894

100

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.583

92.46

0.375
  pilF Thermus thermophilus HB27

37.5

97.666

0.366