Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L92_RS01150 Genome accession   NZ_CP059570
Coordinates   255884..257563 (+) Length   559 a.a.
NCBI ID   WP_085366426.1    Uniprot ID   A0A1X3D5M5
Organism   Neisseria dentiae strain DSM 19151     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250884..262563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L92_RS01125 (H3L92_01125) - 251336..251986 (+) 651 WP_085366418.1 ABC transporter ATP-binding protein -
  H3L92_RS01130 (H3L92_01130) groL 252281..253921 (-) 1641 WP_085366420.1 chaperonin GroEL -
  H3L92_RS01135 (H3L92_01135) groES 254007..254294 (-) 288 WP_085366422.1 co-chaperone GroES -
  H3L92_RS01140 (H3L92_01140) - 254562..255182 (-) 621 WP_085366424.1 isochorismatase family protein -
  H3L92_RS01150 (H3L92_01150) pilF 255884..257563 (+) 1680 WP_085366426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L92_RS01155 (H3L92_01155) pilG 257756..258994 (+) 1239 WP_085366428.1 type II secretion system F family protein Machinery gene
  H3L92_RS01160 (H3L92_01160) pilD 258996..259859 (+) 864 WP_085366430.1 A24 family peptidase Machinery gene
  H3L92_RS01165 (H3L92_01165) coaE 259973..260593 (+) 621 WP_085366432.1 dephospho-CoA kinase -
  H3L92_RS01170 (H3L92_01170) zapD 260590..261348 (+) 759 WP_085366434.1 cell division protein ZapD -
  H3L92_RS01175 (H3L92_01175) yacG 261359..261562 (+) 204 WP_085366436.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62089.71 Da        Isoelectric Point: 6.3563

>NTDB_id=470814 H3L92_RS01150 WP_085366426.1 255884..257563(+) (pilF) [Neisseria dentiae strain DSM 19151]
MSVGILRVLVQSQIVDAAQVERYQSVLKSDKSILPMLFEEGVITPKALGELLARVFSYPLLDLRHYPRSNILSDVLSEEQ
MLQNRCIPIFQRGRKVYFAVSDPTQIQNFQKIAFASGISVDLVVVCDDQLSTLLEWLGQRSTSILNEMSKEHDASAPAQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPACKTPMERPPVPALKKAGFTDEDLAKDWTM
YRAVGCDSCRGKGYKGRAGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=470814 H3L92_RS01150 WP_085366426.1 255884..257563(+) (pilF) [Neisseria dentiae strain DSM 19151]
ATGAGCGTCGGTATATTAAGAGTATTGGTGCAAAGCCAGATTGTCGATGCCGCACAAGTAGAGCGGTATCAAAGCGTATT
AAAAAGCGATAAATCTATTCTTCCTATGTTGTTTGAGGAGGGCGTGATTACACCTAAAGCGCTGGGGGAGTTGTTGGCGC
GGGTGTTCAGCTATCCGCTGTTGGATCTGCGCCATTATCCGCGCAGCAATATCCTTTCGGATGTGCTTTCAGAAGAGCAG
ATGCTGCAAAACCGCTGTATTCCGATTTTTCAGCGGGGTAGAAAAGTTTATTTTGCGGTGTCTGACCCTACCCAGATTCA
AAATTTCCAAAAAATCGCTTTTGCTTCAGGTATATCGGTTGATTTAGTGGTGGTGTGCGACGACCAGCTGAGCACTTTGC
TCGAGTGGTTGGGGCAGCGGTCTACTTCTATTTTGAACGAAATGAGCAAGGAGCATGATGCTTCCGCTCCTGCACAAGCG
TTATATATCGATAATGAAGAGGCGGAAGACGGGCCGATTCCGCGCTTTATCCACAAAACGCTTTCCGATGCATTGAATGC
CGGCGCATCCGATATCCATTTTGAGTTTTACGAACAGATGGCGCGGGTGCGTTTCCGTGTCGACGGCCAGCTTCGGGAGG
TGGTGCAACCTCCGGTGGCGGTGCGCGGCCAGCTGGCTTCGCGCATCAAAGTGATGGCACGTTTGGATATTTCGGAAAAG
CGCGTGCCGCAAGACGGCCGTATTCAAATTGCCTTCCATAAACACGGCCGCCCGATCGATTTCCGTGTCAGCACGCTGCC
TACCTTGTTCGGCGAAAAAGTGGTGATGCGTATTTTGAATTCGGATGCCGGCTCGCTTAATATCGACCAACTCGGTTTCG
AACCTTTTCAAAAAGAAATGCTGCTCGAGGCTATTCACCGTCCTTACGGCATGGTGCTGGTAACCGGCCCCACCGGCTCC
GGTAAAACCGTGTCGCTTTATACCTGTTTGAATATTCTGAATACCGAAAGTGTAAATATTGCTACGGCGGAAGACCCCGC
AGAGATCAATCTGCCCGGCATCAATCAGGTTAACGTGAACGACAAACAGGGGTTAACTTTTGCGGCAGCATTGAAATCGT
TTCTGCGCCAAGACCCGGATATCATTATGGTGGGTGAGATTCGTGATTTGGAAACTGCGGATATTGCCATTAAAGCTGCG
CAAACGGGCCACATGGTATTTTCCACACTGCACACCAATAACGCCCCGGCCACTTTGTCGCGTATGCTGAATATGGGGGT
GGCGCCTTTCAATATCGCCAGTTCGGTCAGCCTGATTATGGCGCAACGTCTGCTGCGCCGTTTGTGCCCGGCATGTAAAA
CGCCGATGGAGCGCCCGCCGGTGCCTGCCTTGAAAAAAGCCGGCTTTACAGATGAAGATCTGGCTAAAGATTGGACGATG
TATCGTGCGGTCGGGTGCGACAGCTGCCGCGGAAAAGGCTATAAAGGCCGTGCCGGTGTGTATGAAGTGATGCCGATTAC
CGAAGAAATGCAGCGGGTGATTATGAATAACGGCACCGAAGTAGATATTATGAATATGGCCTATAAAGAAGGTATGGTGG
ATCTGCGTCGTGCCGGTTTGTTGAAAGTGATGCAGGGGATTACTTCTTTAGAAGAGGTTTTGGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3D5M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

79.249

100

0.792
  pilB Acinetobacter baumannii D1279779

52.244

99.642

0.521
  pilB Acinetobacter baylyi ADP1

50.89

100

0.512
  pilB Legionella pneumophila strain ERS1305867

47.331

100

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.46
  pilB Vibrio cholerae strain A1552

45.374

100

0.456
  pilB Vibrio campbellii strain DS40M4

43.986

99.642

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.623

93.56

0.361