Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H3L95_RS12775 Genome accession   NZ_CP059566
Coordinates   2695148..2696827 (-) Length   559 a.a.
NCBI ID   WP_003757129.1    Uniprot ID   A0A9X5P151
Organism   Neisseria sicca strain DSM 17713     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2690148..2701827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L95_RS12745 (H3L95_12745) - 2690404..2691255 (-) 852 WP_003757138.1 mechanosensitive ion channel family protein -
  H3L95_RS12750 (H3L95_12750) - 2691559..2691894 (+) 336 WP_003757137.1 hypothetical protein -
  H3L95_RS12755 (H3L95_12755) yacG 2692099..2692284 (-) 186 WP_040668227.1 DNA gyrase inhibitor YacG -
  H3L95_RS12760 (H3L95_12760) coaE 2692339..2692968 (-) 630 WP_003757134.1 dephospho-CoA kinase -
  H3L95_RS12765 (H3L95_12765) pilD 2692970..2693833 (-) 864 WP_003757133.1 A24 family peptidase Machinery gene
  H3L95_RS12770 (H3L95_12770) pilG 2693835..2695076 (-) 1242 WP_003757131.1 type II secretion system F family protein Machinery gene
  H3L95_RS12775 (H3L95_12775) pilF 2695148..2696827 (-) 1680 WP_003757129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H3L95_RS12780 (H3L95_12780) - 2697352..2697825 (-) 474 WP_003757128.1 outer membrane protein assembly factor BamE -
  H3L95_RS12785 (H3L95_12785) - 2697890..2699149 (-) 1260 WP_003757126.1 YadA family autotransporter adhesin -
  H3L95_RS12790 (H3L95_12790) apbC 2699878..2700957 (-) 1080 WP_003757122.1 iron-sulfur cluster carrier protein ApbC -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62225.71 Da        Isoelectric Point: 6.4397

>NTDB_id=470757 H3L95_RS12775 WP_003757129.1 2695148..2696827(-) (pilF) [Neisseria sicca strain DSM 17713]
MSIGLLRVLLQSQTITNAQAEHYGNALKSGKEILPMLFADGIISPRSLGELVARVFSYPLLDLHHYPRSNVITDVLSEEQ
MVQNRCIPIFRRGRKVYLAVSDPTQIQNFQKITFSSGVTIDLVVVPDDQLSSLLEWLGQRSTTILKEISEEQEAAQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKREVERPPVPALKKAGFTDEDLAKDWKL
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIMNMAYKEGMVDLRRAGLLKVMQGLTSLEEVIAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=470757 H3L95_RS12775 WP_003757129.1 2695148..2696827(-) (pilF) [Neisseria sicca strain DSM 17713]
ATGAGTATCGGACTATTACGCGTACTGCTTCAAAGCCAGACCATTACAAATGCACAAGCTGAACATTACGGCAATGCCTT
GAAATCAGGGAAAGAAATCTTACCCATGCTTTTTGCGGACGGCATCATCTCTCCTAGAAGTTTAGGAGAATTAGTTGCAC
GTGTATTCAGCTACCCCTTATTGGATTTGCATCATTATCCTCGCAGCAATGTCATAACTGATGTTTTAAGCGAAGAACAG
ATGGTTCAAAATCGATGCATACCCATATTTCGTCGCGGCCGTAAAGTTTATCTGGCCGTATCAGACCCGACACAAATCCA
AAACTTCCAAAAAATCACTTTTTCTTCAGGTGTTACGATAGATTTGGTTGTCGTACCTGACGATCAGTTAAGCTCCCTTT
TAGAATGGTTAGGACAACGTTCTACCACCATCTTAAAAGAAATTAGTGAAGAACAAGAAGCTGCCCAGCCCTCTCAGGCG
CTTTATATTGACAACGAAGAGGCTGAAGATGGACCTATTCCACGCTTTATTCACAAAACTTTATCAGATGCACTAAATGC
AGGCGCATCCGATATACATTTCGAATTTTATGAACAAATGGCTCGTGTCCGTTTTCGTGTAGATGGACAGCTAAGGGAAG
TGGTTCAACCCCCTGTTGCTGTACGCGGACAGCTGGCTTCACGCATCAAAGTAATGGCGCGTTTGGACATTTCGGAAAAA
CGCGTACCGCAAGATGGTCGTATTCAGATTGCTTTCCATAAACACGGTCGCCCCATTGATTTTCGTGTAAGTACGTTACC
TACGCTTTTTGGTGAAAAAGTGGTAATGCGTATTCTGAATTCTGATGCAGCAACTTTAAATATTGACCAACTGGGATTCG
AACCCTTCCAAAAAGAAATGCTGCTTGAGGCCATTCATCGGCCTTATGGCATGGTATTGGTAACAGGTCCGACAGGCTCC
GGTAAAACCGTATCACTTTATACTTGCTTGAATATTTTAAATACAGAAGACGTCAATATTTCCACCGCAGAAGACCCGGC
CGAGATTAATTTGCCCGGCATCAACCAAGTCAACGTCAATGATAAACAAGGTTTGACTTTTGCTGCGGCGTTGAAATCTT
TCTTGCGACAAGACCCTGACATTATCATGGTAGGTGAGATTCGGGACTTGGAAACCGCGGATATTGCCATCAAAGCGGCA
CAAACCGGACATATGGTGTTTTCAACGTTGCATACCAATAATGCGCCTGCGACCCTATCCCGTATGTTGAACATGGGGGT
TGCCCCATTCAACATTGCCAGTTCCGTCAGCTTAATTATGGCGCAACGACTATTGCGTCGTCTGTGTTCGAGCTGTAAGC
GTGAGGTTGAACGTCCTCCCGTTCCCGCATTAAAAAAAGCAGGATTTACAGATGAAGATTTAGCAAAGGATTGGAAACTC
TACCGTCCTGTCGGTTGCGATAGTTGTCGTGGTAAAGGCTTTAAAGGCCGTGTCGGTGTCTACGAAGTAATGCCGATTAC
CGAGGAAATGCAACGTGTCATTATGAATAATGGTACGGAAGTTGATATTATGAACATGGCCTATAAAGAAGGCATGGTCG
ATTTACGCCGTGCAGGCTTATTAAAAGTTATGCAAGGGTTGACTTCATTAGAAGAAGTTATTGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

80.143

100

0.801
  pilB Acinetobacter baylyi ADP1

51.146

100

0.519
  pilB Acinetobacter baumannii D1279779

51.439

99.463

0.512
  pilB Legionella pneumophila strain ERS1305867

49.434

94.812

0.469
  pilB Vibrio cholerae strain A1552

45.552

100

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.035

100

0.454
  pilB Vibrio campbellii strain DS40M4

43.44

100

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.722

95.17

0.369