Detailed information NTG0470147

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: H2N74_RS12605
• Genome accession: NZ_CP059495
• Coordinates: 2606686..2607300 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain JSRB 166
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2601686..2612300

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2N74_RS12575 (H2N74_12575)	rpsT	2601989..2602255 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
H2N74_RS12580 (H2N74_12580)	holA	2602218..2603313 (-)	1096	Protein_2456	DNA polymerase III subunit delta	-
H2N74_RS12585 (H2N74_12585)	-	2603353..2603504 (-)	152	Protein_2457	hypothetical protein	-
H2N74_RS12590 (H2N74_12590)	-	2603545..2603679 (+)	135	WP_003152870.1	YqzM family protein	-
H2N74_RS12595 (H2N74_12595)	comEC	2603698..2606049 (-)	2352	WP_182071847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H2N74_RS12600 (H2N74_12600)	-	2606050..2606619 (-)	570	WP_003152868.1	ComE operon protein 2	-
H2N74_RS12605 (H2N74_12605)	comEA	2606686..2607300 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
H2N74_RS12610 (H2N74_12610)	comER	2607359..2608180 (+)	822	WP_012118027.1	late competence protein ComER	-
H2N74_RS12615 (H2N74_12615)	-	2608249..2608986 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
H2N74_RS12620 (H2N74_12620)	rsfS	2608983..2609339 (-)	357	WP_007408260.1	ribosome silencing factor	-
H2N74_RS12625 (H2N74_12625)	yqeK	2609357..2609917 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H2N74_RS12630 (H2N74_12630)	-	2609907..2610476 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
H2N74_RS12635 (H2N74_12635)	yhbY	2610487..2610777 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
H2N74_RS12640 (H2N74_12640)	aroE	2610771..2611613 (-)	843	WP_182071849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=470147 H2N74_RS12605 WP_014418419.1 2606686..2607300(-) (comEA) [Bacillus velezensis strain JSRB 166]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=470147 H2N74_RS12605 WP_014418419.1 2606686..2607300(-) (comEA) [Bacillus velezensis strain JSRB 166]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363