Detailed information NTG0470145

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: H2N74_RS12595
• Genome accession: NZ_CP059495
• Coordinates: 2603698..2606049 (-)
• Length: 783 a.a.
• NCBI ID: WP_182071847.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JSRB 166
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2598698..2611049

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2N74_RS12560 (H2N74_12560)	-	2599064..2599402 (-)	339	WP_007408270.1	YqxA family protein	-
H2N74_RS12565 (H2N74_12565)	spoIIP	2599419..2600612 (-)	1194	WP_007612673.1	stage II sporulation protein P	-
H2N74_RS12570 (H2N74_12570)	gpr	2600680..2601786 (-)	1107	WP_014418417.1	GPR endopeptidase	-
H2N74_RS12575 (H2N74_12575)	rpsT	2601989..2602255 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
H2N74_RS12580 (H2N74_12580)	holA	2602218..2603313 (-)	1096	Protein_2456	DNA polymerase III subunit delta	-
H2N74_RS12585 (H2N74_12585)	-	2603353..2603504 (-)	152	Protein_2457	hypothetical protein	-
H2N74_RS12590 (H2N74_12590)	-	2603545..2603679 (+)	135	WP_003152870.1	YqzM family protein	-
H2N74_RS12595 (H2N74_12595)	comEC	2603698..2606049 (-)	2352	WP_182071847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H2N74_RS12600 (H2N74_12600)	-	2606050..2606619 (-)	570	WP_003152868.1	ComE operon protein 2	-
H2N74_RS12605 (H2N74_12605)	comEA	2606686..2607300 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
H2N74_RS12610 (H2N74_12610)	comER	2607359..2608180 (+)	822	WP_012118027.1	late competence protein ComER	-
H2N74_RS12615 (H2N74_12615)	-	2608249..2608986 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
H2N74_RS12620 (H2N74_12620)	rsfS	2608983..2609339 (-)	357	WP_007408260.1	ribosome silencing factor	-
H2N74_RS12625 (H2N74_12625)	yqeK	2609357..2609917 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H2N74_RS12630 (H2N74_12630)	-	2609907..2610476 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
H2N74_RS12635 (H2N74_12635)	yhbY	2610487..2610777 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86824.74 Da
• Isoelectric Point: 9.0097

>NTDB_id=470145 H2N74_RS12595 WP_182071847.1 2603698..2606049(-) (comEC) [Bacillus velezensis strain JSRB 166]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQHAPVIVCLVSFCLYFFLYTVCDATNVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWTASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFIIYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCTVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGIVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=470145 H2N74_RS12595 WP_182071847.1 2603698..2606049(-) (comEC) [Bacillus velezensis strain JSRB 166]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTACGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GACGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGTTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATTGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTATATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACTCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCACGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGATCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.995	98.595	0.562