Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOLKNPGA_RS09230 Genome accession   NZ_CP059400
Coordinates   2099947..2101665 (-) Length   572 a.a.
NCBI ID   WP_182349642.1    Uniprot ID   -
Organism   Legionella sp. PC1000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2094947..2106665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOLKNPGA_RS09210 (FOLKNPGA_01873) - 2095821..2097005 (-) 1185 WP_182349638.1 aminotransferase class V-fold PLP-dependent enzyme -
  FOLKNPGA_RS09215 (FOLKNPGA_01874) - 2097037..2097804 (-) 768 WP_182349639.1 HAD family hydrolase -
  FOLKNPGA_RS09220 (FOLKNPGA_01875) - 2097826..2098686 (-) 861 WP_182349640.1 A24 family peptidase -
  FOLKNPGA_RS09225 (FOLKNPGA_01876) pilC 2098716..2099936 (-) 1221 WP_182349641.1 type II secretion system F family protein Machinery gene
  FOLKNPGA_RS09230 (FOLKNPGA_01877) pilB 2099947..2101665 (-) 1719 WP_182349642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOLKNPGA_RS09235 (FOLKNPGA_01878) - 2101919..2102710 (+) 792 WP_182349643.1 methyltransferase domain-containing protein -
  FOLKNPGA_RS09240 (FOLKNPGA_01879) - 2102811..2103392 (-) 582 WP_182349644.1 hypothetical protein -
  FOLKNPGA_RS09245 (FOLKNPGA_01880) - 2103573..2103899 (+) 327 WP_182349645.1 DUF1820 family protein -
  FOLKNPGA_RS09250 (FOLKNPGA_01881) - 2103945..2104505 (+) 561 WP_182349646.1 hypoxanthine-guanine phosphoribosyltransferase -
  FOLKNPGA_RS09255 (FOLKNPGA_01882) - 2105081..2105965 (+) 885 WP_182349647.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63012.69 Da        Isoelectric Point: 6.3297

>NTDB_id=469382 FOLKNPGA_RS09230 WP_182349642.1 2099947..2101665(-) (pilB) [Legionella sp. PC1000]
MLGSNEFKLHGIGQLFVLEKLLDKTEALEFCKNASAEKISLVQYLVKNNILSATQIAFTAAHNFGVPMLDLDCIDIDSIP
INLVNEKLIRRHSMVPLFYRGNNLYLATEDPSKQASLKEIQFHTGLNTHAIIVEADKLSTLIDHLLTVQETQGLSEFVGD
TTEFDGIIINEEEDHETSIAPSITEDAPIVKFVNKILLDAIKQGASDIHFEPYEKEYRIRYRQDGILHEIATPPGSLSTR
ITARIKIMSSLDISERRIPQDGGFKMKISKTRSIDFRVSTCPTTSGEKVVMRILDPGSAKLGIEALGFSSLQKEQFVHAI
ERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKVPGINQVNINPKAGLTFSNALRSFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNSAADTLNRLVNMGLATFNVASSVTLIIAQRLARKLCEHCKVLRDDFNKPGLL
ELGFAEADLKEIKLYKAGGCAKCTNGYRGRIGLFEVLPMTKKLGQIIMSGGNSLDILRIAKEEGMITIYQSGLEKVKQGI
TTIEEVNRVTVD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=469382 FOLKNPGA_RS09230 WP_182349642.1 2099947..2101665(-) (pilB) [Legionella sp. PC1000]
ATGTTAGGAAGCAATGAATTCAAATTACATGGGATAGGACAGTTGTTTGTTCTTGAAAAGCTTTTGGATAAAACAGAGGC
GCTTGAATTCTGCAAGAATGCTTCCGCGGAAAAAATATCCTTAGTTCAATACCTTGTCAAAAATAACATTTTATCAGCCA
CACAAATTGCATTTACAGCAGCACATAATTTCGGCGTTCCTATGCTTGATTTGGACTGTATTGATATCGATTCCATACCT
ATCAATTTGGTCAATGAAAAGCTAATTCGTCGCCACTCCATGGTTCCTTTATTTTATCGTGGAAATAATTTATATCTTGC
AACAGAAGACCCAAGCAAACAAGCTTCTTTAAAAGAAATCCAATTCCATACCGGACTCAATACCCATGCAATTATAGTTG
AAGCAGATAAACTTAGCACATTAATTGATCATTTACTTACAGTACAAGAAACTCAGGGCTTATCTGAATTTGTTGGGGAT
ACTACTGAATTTGATGGAATTATTATTAATGAGGAAGAAGACCATGAAACAAGTATTGCTCCCTCAATAACAGAAGATGC
TCCTATAGTTAAATTCGTTAATAAAATACTTCTTGATGCGATTAAACAAGGAGCTTCAGACATCCATTTTGAACCCTATG
AAAAAGAATATCGTATACGGTATCGACAAGATGGTATTTTGCATGAAATCGCAACGCCTCCTGGAAGCTTATCCACACGC
ATTACCGCACGAATAAAAATCATGTCCAGTCTGGATATTTCAGAAAGACGAATTCCACAAGATGGTGGTTTTAAGATGAA
AATATCCAAAACCCGATCCATTGATTTTCGTGTAAGCACCTGCCCTACTACTTCAGGTGAAAAAGTCGTGATGCGGATAT
TAGATCCAGGTTCAGCAAAATTGGGTATTGAAGCCTTAGGTTTCAGTTCACTTCAAAAAGAACAATTTGTCCATGCAATT
GAACGTCCCCAAGGAATGATCCTGGTCACTGGACCAACAGGCAGTGGTAAAACCGTGACCTTATACACTGCATTGAATAT
CCTCAATACTAAAGAAGTAAATATTTCCACTGCTGAAGACCCTGTTGAAATTAAAGTACCAGGAATCAATCAGGTTAATA
TCAATCCCAAAGCGGGACTCACTTTTTCCAATGCATTACGGTCATTTTTACGGCAAGACCCTGACATCATAATGGTTGGG
GAAATACGTGATTTGGAAACAGCTGAAATAGCAGTTAAAGCCGCACAAACAGGACATTTGGTACTCTCAACGTTACATAC
GAATAGCGCTGCTGATACATTAAACCGTTTGGTCAATATGGGACTCGCAACCTTTAATGTCGCAAGCTCAGTAACGCTTA
TTATCGCACAACGCTTAGCCCGAAAACTATGTGAACATTGTAAAGTCTTAAGAGATGATTTCAACAAACCGGGTTTGTTG
GAATTAGGCTTTGCAGAAGCTGATTTAAAGGAAATTAAATTATATAAAGCTGGAGGATGCGCCAAGTGTACCAATGGATA
CCGTGGGAGAATAGGATTGTTTGAGGTATTGCCTATGACTAAAAAACTGGGTCAAATCATCATGTCGGGAGGAAATTCTT
TGGATATATTAAGAATTGCCAAAGAAGAAGGAATGATTACTATTTATCAATCGGGGCTTGAGAAAGTGAAACAAGGCATT
ACCACTATAGAGGAAGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

79.895

100

0.799
  pilB Acinetobacter baylyi ADP1

51.389

100

0.517
  pilB Acinetobacter baumannii D1279779

53.102

95.804

0.509
  pilB Vibrio cholerae strain A1552

48.276

96.329

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

47.473

96.853

0.46
  pilB Vibrio campbellii strain DS40M4

48.052

94.231

0.453
  pilF Neisseria gonorrhoeae MS11

46.909

96.154

0.451
  pilF Thermus thermophilus HB27

39.092

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.483

0.365