Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H1C99_RS06075 Genome accession   NZ_CP059331
Coordinates   1225757..1227163 (+) Length   468 a.a.
NCBI ID   WP_240030393.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0597     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1220757..1232163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1C99_RS06045 (H1C99_06025) - 1220832..1221596 (-) 765 WP_240030392.1 TSUP family transporter -
  H1C99_RS06050 (H1C99_06030) proB 1221596..1222687 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  H1C99_RS06055 (H1C99_06035) folA 1222799..1223293 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1C99_RS06060 (H1C99_06040) - 1223412..1223642 (+) 231 Protein_1160 YgjV family protein -
  H1C99_RS06065 (H1C99_06045) radA 1223697..1225076 (-) 1380 WP_240027733.1 DNA repair protein RadA -
  H1C99_RS06070 (H1C99_06050) pilA 1225257..1225736 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  H1C99_RS06075 (H1C99_06055) pilB 1225757..1227163 (+) 1407 WP_240030393.1 GspE/PulE family protein Machinery gene
  H1C99_RS06080 (H1C99_06060) - 1227147..1228341 (+) 1195 Protein_1164 type II secretion system F family protein -
  H1C99_RS12990 - 1228620..1228826 (+) 207 Protein_1165 peptidase A24 -
  H1C99_RS06085 (H1C99_06070) coaE 1229040..1229675 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  H1C99_RS06090 (H1C99_06075) yacG 1229657..1229842 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1C99_RS06095 (H1C99_06080) holA 1229843..1230871 (-) 1029 WP_240030433.1 DNA polymerase III subunit delta -
  H1C99_RS06100 (H1C99_06085) lptE 1230914..1231402 (-) 489 WP_240027735.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 53074.27 Da        Isoelectric Point: 5.6595

>NTDB_id=468770 H1C99_RS06075 WP_240030393.1 1225757..1227163(+) (pilB) [Mannheimia haemolytica strain 0597]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTXGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRT
FLRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCG
GAGCEHCYQGYKGRIGVYQLLNRSAKNFXKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=468770 H1C99_RS06075 WP_240030393.1 1225757..1227163(+) (pilB) [Mannheimia haemolytica strain 0597]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACANNGGGAAG
CGGTAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGA
TTGAAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACC
TTTCTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGC
ACAAACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCA
TAAAAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGT
GGGGCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCNAAAAA
TTTTGANAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCA
ATCAGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.294

98.718

0.714
  pilB Haemophilus influenzae Rd KW20

56.587

98.932

0.56
  pilB Haemophilus influenzae 86-028NP

55.819

99.145

0.553
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.417
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.415
  pilB Vibrio cholerae strain A1552

39.959

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

38.477

100

0.41
  pilB Acinetobacter baylyi ADP1

35.857

100

0.385
  pilB Acinetobacter baumannii D1279779

36.327

100

0.38