Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1C99_RS06070 Genome accession   NZ_CP059331
Coordinates   1225257..1225736 (+) Length   159 a.a.
NCBI ID   WP_020831302.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0597     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1220257..1230736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1C99_RS06045 (H1C99_06025) - 1220832..1221596 (-) 765 WP_240030392.1 TSUP family transporter -
  H1C99_RS06050 (H1C99_06030) proB 1221596..1222687 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  H1C99_RS06055 (H1C99_06035) folA 1222799..1223293 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1C99_RS06060 (H1C99_06040) - 1223412..1223642 (+) 231 Protein_1160 YgjV family protein -
  H1C99_RS06065 (H1C99_06045) radA 1223697..1225076 (-) 1380 WP_240027733.1 DNA repair protein RadA -
  H1C99_RS06070 (H1C99_06050) pilA 1225257..1225736 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  H1C99_RS06075 (H1C99_06055) pilB 1225757..1227163 (+) 1407 WP_240030393.1 GspE/PulE family protein Machinery gene
  H1C99_RS06080 (H1C99_06060) - 1227147..1228341 (+) 1195 Protein_1164 type II secretion system F family protein -
  H1C99_RS12990 - 1228620..1228826 (+) 207 Protein_1165 peptidase A24 -
  H1C99_RS06085 (H1C99_06070) coaE 1229040..1229675 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  H1C99_RS06090 (H1C99_06075) yacG 1229657..1229842 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16855.17 Da        Isoelectric Point: 8.7964

>NTDB_id=468769 H1C99_RS06070 WP_020831302.1 1225257..1225736(+) (pilA) [Mannheimia haemolytica strain 0597]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468769 H1C99_RS06070 WP_020831302.1 1225257..1225736(+) (pilA) [Mannheimia haemolytica strain 0597]
ATGCAAAAAATTGTACATTTTCGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTAATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

57.143

88.05

0.503
  pilA Haemophilus influenzae Rd KW20

55.634

89.308

0.497