Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H1D03_RS01475 Genome accession   NZ_CP059327
Coordinates   294977..296380 (+) Length   467 a.a.
NCBI ID   WP_240027734.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0729     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289977..301380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D03_RS01445 (H1D03_01445) - 290053..290817 (-) 765 WP_240028779.1 TSUP family transporter -
  H1D03_RS01450 (H1D03_01450) proB 290817..291908 (-) 1092 WP_240027731.1 glutamate 5-kinase -
  H1D03_RS01455 (H1D03_01455) folA 292020..292514 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1D03_RS01460 (H1D03_01460) - 292633..292863 (+) 231 WP_006251345.1 YgjV family protein -
  H1D03_RS01465 (H1D03_01465) radA 292918..294297 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  H1D03_RS01470 (H1D03_01470) pilA 294549..294956 (+) 408 WP_006251342.1 pilin Machinery gene
  H1D03_RS01475 (H1D03_01475) pilB 294977..296380 (+) 1404 WP_240027734.1 GspE/PulE family protein Machinery gene
  H1D03_RS01480 (H1D03_01480) - 296364..297558 (+) 1195 Protein_296 type II secretion system F family protein -
  H1D03_RS01485 (H1D03_01490) coaE 298259..298894 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  H1D03_RS01490 (H1D03_01495) yacG 298876..299061 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D03_RS01495 (H1D03_01500) holA 299062..300087 (-) 1026 WP_240029043.1 DNA polymerase III subunit delta -
  H1D03_RS01500 (H1D03_01505) lptE 300130..300618 (-) 489 WP_006251337.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52955.39 Da        Isoelectric Point: 5.6595

>NTDB_id=468668 H1D03_RS01475 WP_240027734.1 294977..296380(+) (pilB) [Mannheimia haemolytica strain 0729]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGRIGVYQLLNRSAKNFXKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=468668 H1D03_RS01475 WP_240027734.1 294977..296380(+) (pilB) [Mannheimia haemolytica strain 0729]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGGAAGCGG
TAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCNAAAAATTT
TGANAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATC
AGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.451

98.715

0.715
  pilB Haemophilus influenzae Rd KW20

56.71

98.929

0.561
  pilB Haemophilus influenzae 86-028NP

55.94

99.143

0.555
  pilB Vibrio campbellii strain DS40M4

40.541

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.754

100

0.415
  pilB Vibrio cholerae strain A1552

40.041

100

0.413
  pilB Vibrio parahaemolyticus RIMD 2210633

38.554

100

0.411
  pilB Acinetobacter baylyi ADP1

35.928

100

0.385
  pilB Acinetobacter baumannii D1279779

36.401

100

0.381