Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D03_RS01470 Genome accession   NZ_CP059327
Coordinates   294549..294956 (+) Length   135 a.a.
NCBI ID   WP_006251342.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0729     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 289549..299956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D03_RS01445 (H1D03_01445) - 290053..290817 (-) 765 WP_240028779.1 TSUP family transporter -
  H1D03_RS01450 (H1D03_01450) proB 290817..291908 (-) 1092 WP_240027731.1 glutamate 5-kinase -
  H1D03_RS01455 (H1D03_01455) folA 292020..292514 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  H1D03_RS01460 (H1D03_01460) - 292633..292863 (+) 231 WP_006251345.1 YgjV family protein -
  H1D03_RS01465 (H1D03_01465) radA 292918..294297 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  H1D03_RS01470 (H1D03_01470) pilA 294549..294956 (+) 408 WP_006251342.1 pilin Machinery gene
  H1D03_RS01475 (H1D03_01475) pilB 294977..296380 (+) 1404 WP_240027734.1 GspE/PulE family protein Machinery gene
  H1D03_RS01480 (H1D03_01480) - 296364..297558 (+) 1195 Protein_296 type II secretion system F family protein -
  H1D03_RS01485 (H1D03_01490) coaE 298259..298894 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  H1D03_RS01490 (H1D03_01495) yacG 298876..299061 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13955.63 Da        Isoelectric Point: 7.5809

>NTDB_id=468667 H1D03_RS01470 WP_006251342.1 294549..294956(+) (pilA) [Mannheimia haemolytica strain 0729]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=468667 H1D03_RS01470 WP_006251342.1 294549..294956(+) (pilA) [Mannheimia haemolytica strain 0729]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.327

83.704

0.43
  pilA Haemophilus influenzae Rd KW20

50

84.444

0.422