Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H1D06_RS01075 Genome accession   NZ_CP059324
Coordinates   217138..218544 (-) Length   468 a.a.
NCBI ID   WP_240018336.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0760     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 212138..223544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D06_RS01045 (H1D06_01035) lptE 212907..213395 (+) 489 WP_006251337.1 LPS assembly lipoprotein LptE -
  H1D06_RS01050 (H1D06_01040) holA 213438..214463 (+) 1026 WP_240018837.1 DNA polymerase III subunit delta -
  H1D06_RS01055 (H1D06_01045) yacG 214464..214649 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D06_RS01060 (H1D06_01050) coaE 214631..215266 (-) 636 WP_240018838.1 dephospho-CoA kinase -
  H1D06_RS12905 - 215477..215683 (-) 207 Protein_204 peptidase A24 -
  H1D06_RS01065 - 215754..215957 (-) 204 WP_240018335.1 hypothetical protein -
  H1D06_RS01070 (H1D06_01060) - 215960..217154 (-) 1195 Protein_206 type II secretion system F family protein -
  H1D06_RS01075 (H1D06_01065) pilB 217138..218544 (-) 1407 WP_240018336.1 GspE/PulE family protein Machinery gene
  H1D06_RS01080 (H1D06_01070) pilA 218565..218972 (-) 408 WP_006251342.1 pilin Machinery gene
  H1D06_RS01085 (H1D06_01075) radA 219224..220603 (+) 1380 WP_240018337.1 DNA repair protein RadA -
  H1D06_RS01090 (H1D06_01080) - 220658..220888 (-) 231 WP_006251345.1 YgjV family protein -
  H1D06_RS01095 (H1D06_01085) - 221007..221501 (-) 495 WP_240018338.1 dihydrofolate reductase -
  H1D06_RS01100 (H1D06_01090) proB 221613..222704 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  H1D06_RS01105 (H1D06_01095) - 222704..223468 (+) 765 WP_240018340.1 TSUP family transporter -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 53065.93 Da        Isoelectric Point: 5.2465

>NTDB_id=468599 H1D06_RS01075 WP_240018336.1 217138..218544(-) (pilB) [Mannheimia haemolytica strain 0760]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTXGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRT
FLRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCG
GAGCEHCYQGYKGRIGVYQLLNRSAXNFEXNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=468599 H1D06_RS01075 WP_240018336.1 217138..218544(-) (pilB) [Mannheimia haemolytica strain 0760]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGNNGGAAG
CGGTAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGA
TTGAAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACC
TTTCTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGC
ACAAACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCA
TAAAAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGT
GGGGCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCANAAAA
TTTTGAANAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCA
ATCAGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

71.861

98.718

0.709
  pilB Haemophilus influenzae Rd KW20

56.587

98.932

0.56
  pilB Haemophilus influenzae 86-028NP

55.819

99.145

0.553
  pilB Vibrio cholerae strain A1552

40.329

100

0.419
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.417
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

38.477

100

0.41
  pilB Acinetobacter baylyi ADP1

35.857

100

0.385
  pilB Acinetobacter baumannii D1279779

36.327

100

0.38