Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D06_RS01080 Genome accession   NZ_CP059324
Coordinates   218565..218972 (-) Length   135 a.a.
NCBI ID   WP_006251342.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0760     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 213565..223972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D06_RS01055 (H1D06_01045) yacG 214464..214649 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D06_RS01060 (H1D06_01050) coaE 214631..215266 (-) 636 WP_240018838.1 dephospho-CoA kinase -
  H1D06_RS12905 - 215477..215683 (-) 207 Protein_204 peptidase A24 -
  H1D06_RS01065 - 215754..215957 (-) 204 WP_240018335.1 hypothetical protein -
  H1D06_RS01070 (H1D06_01060) - 215960..217154 (-) 1195 Protein_206 type II secretion system F family protein -
  H1D06_RS01075 (H1D06_01065) pilB 217138..218544 (-) 1407 WP_240018336.1 GspE/PulE family protein Machinery gene
  H1D06_RS01080 (H1D06_01070) pilA 218565..218972 (-) 408 WP_006251342.1 pilin Machinery gene
  H1D06_RS01085 (H1D06_01075) radA 219224..220603 (+) 1380 WP_240018337.1 DNA repair protein RadA -
  H1D06_RS01090 (H1D06_01080) - 220658..220888 (-) 231 WP_006251345.1 YgjV family protein -
  H1D06_RS01095 (H1D06_01085) - 221007..221501 (-) 495 WP_240018338.1 dihydrofolate reductase -
  H1D06_RS01100 (H1D06_01090) proB 221613..222704 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  H1D06_RS01105 (H1D06_01095) - 222704..223468 (+) 765 WP_240018340.1 TSUP family transporter -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13955.63 Da        Isoelectric Point: 7.5809

>NTDB_id=468600 H1D06_RS01080 WP_006251342.1 218565..218972(-) (pilA) [Mannheimia haemolytica strain 0760]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=468600 H1D06_RS01080 WP_006251342.1 218565..218972(-) (pilA) [Mannheimia haemolytica strain 0760]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.327

83.704

0.43
  pilA Haemophilus influenzae Rd KW20

50

84.444

0.422