Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H1D07_RS06090 Genome accession   NZ_CP059323
Coordinates   1255594..1256997 (-) Length   467 a.a.
NCBI ID   WP_240027271.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0768     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1250594..1261997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D07_RS06065 (H1D07_06100) lptE 1251361..1251849 (+) 489 WP_006251337.1 LPS assembly lipoprotein LptE -
  H1D07_RS06070 (H1D07_06105) holA 1251892..1252917 (+) 1026 WP_240018837.1 DNA polymerase III subunit delta -
  H1D07_RS06075 (H1D07_06110) yacG 1252918..1253103 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D07_RS06080 (H1D07_06115) coaE 1253085..1253720 (-) 636 WP_240018838.1 dephospho-CoA kinase -
  H1D07_RS12925 (H1D07_06120) - 1253934..1254413 (-) 480 Protein_1192 peptidase A24 -
  H1D07_RS06085 (H1D07_06125) - 1254416..1255610 (-) 1195 Protein_1193 type II secretion system F family protein -
  H1D07_RS06090 (H1D07_06130) pilB 1255594..1256997 (-) 1404 WP_240027271.1 GspE/PulE family protein Machinery gene
  H1D07_RS06095 (H1D07_06135) pilA 1257018..1257497 (-) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  H1D07_RS06100 (H1D07_06140) radA 1257678..1259057 (+) 1380 WP_240018337.1 DNA repair protein RadA -
  H1D07_RS06105 (H1D07_06145) - 1259112..1259342 (-) 231 WP_240027272.1 YgjV family protein -
  H1D07_RS06110 (H1D07_06150) - 1259461..1259955 (-) 495 WP_240018338.1 dihydrofolate reductase -
  H1D07_RS06115 (H1D07_06155) proB 1260067..1261158 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  H1D07_RS06120 (H1D07_06160) - 1261158..1261922 (+) 765 WP_240020921.1 TSUP family transporter -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52947.04 Da        Isoelectric Point: 5.2465

>NTDB_id=468586 H1D07_RS06090 WP_240027271.1 1255594..1256997(-) (pilB) [Mannheimia haemolytica strain 0768]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGRIGVYQLLNRSAXNFEXNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=468586 H1D07_RS06090 WP_240027271.1 1255594..1256997(-) (pilB) [Mannheimia haemolytica strain 0768]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGGAAGCGG
TAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCANAAAATTT
TGAANAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATC
AGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.017

98.715

0.711
  pilB Haemophilus influenzae Rd KW20

56.71

98.929

0.561
  pilB Haemophilus influenzae 86-028NP

55.94

99.143

0.555
  pilB Vibrio cholerae strain A1552

40.412

100

0.42
  pilB Vibrio campbellii strain DS40M4

40.541

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.754

100

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

38.554

100

0.411
  pilB Acinetobacter baylyi ADP1

35.928

100

0.385
  pilB Acinetobacter baumannii D1279779

36.401

100

0.381