Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H1D07_RS06095 Genome accession   NZ_CP059323
Coordinates   1257018..1257497 (-) Length   159 a.a.
NCBI ID   WP_020831302.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0768     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1252018..1262497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D07_RS06075 (H1D07_06110) yacG 1252918..1253103 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D07_RS06080 (H1D07_06115) coaE 1253085..1253720 (-) 636 WP_240018838.1 dephospho-CoA kinase -
  H1D07_RS12925 (H1D07_06120) - 1253934..1254413 (-) 480 Protein_1192 peptidase A24 -
  H1D07_RS06085 (H1D07_06125) - 1254416..1255610 (-) 1195 Protein_1193 type II secretion system F family protein -
  H1D07_RS06090 (H1D07_06130) pilB 1255594..1256997 (-) 1404 WP_240027271.1 GspE/PulE family protein Machinery gene
  H1D07_RS06095 (H1D07_06135) pilA 1257018..1257497 (-) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  H1D07_RS06100 (H1D07_06140) radA 1257678..1259057 (+) 1380 WP_240018337.1 DNA repair protein RadA -
  H1D07_RS06105 (H1D07_06145) - 1259112..1259342 (-) 231 WP_240027272.1 YgjV family protein -
  H1D07_RS06110 (H1D07_06150) - 1259461..1259955 (-) 495 WP_240018338.1 dihydrofolate reductase -
  H1D07_RS06115 (H1D07_06155) proB 1260067..1261158 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  H1D07_RS06120 (H1D07_06160) - 1261158..1261922 (+) 765 WP_240020921.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16855.17 Da        Isoelectric Point: 8.7964

>NTDB_id=468587 H1D07_RS06095 WP_020831302.1 1257018..1257497(-) (pilA) [Mannheimia haemolytica strain 0768]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468587 H1D07_RS06095 WP_020831302.1 1257018..1257497(-) (pilA) [Mannheimia haemolytica strain 0768]
ATGCAAAAAATTGTACATTTTCGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTAATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

57.143

88.05

0.503
  pilA Haemophilus influenzae Rd KW20

55.634

89.308

0.497