Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HS060_RS06800 Genome accession   NZ_CP059238
Coordinates   1390087..1391490 (-) Length   467 a.a.
NCBI ID   WP_240020920.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0152     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1385087..1396490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS060_RS06765 (HS060_06760) lptE 1385852..1386340 (+) 489 WP_240020919.1 LPS assembly lipoprotein LptE -
  HS060_RS06770 (HS060_06765) holA 1386383..1387408 (+) 1026 WP_409035839.1 DNA polymerase III subunit delta -
  HS060_RS06775 (HS060_06770) yacG 1387409..1387594 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS060_RS06780 (HS060_06775) coaE 1387576..1388211 (-) 636 WP_020831305.1 dephospho-CoA kinase -
  HS060_RS06785 - 1388425..1388631 (-) 207 Protein_1312 peptidase A24 -
  HS060_RS06790 - 1388702..1388905 (-) 204 WP_240018335.1 hypothetical protein -
  HS060_RS06795 (HS060_06785) - 1388908..1390103 (-) 1196 Protein_1314 type II secretion system F family protein -
  HS060_RS06800 (HS060_06790) pilB 1390087..1391490 (-) 1404 WP_240020920.1 GspE/PulE family protein Machinery gene
  HS060_RS06805 (HS060_06795) pilA 1391511..1391990 (-) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  HS060_RS06810 (HS060_06800) radA 1392171..1393553 (+) 1383 WP_409035840.1 DNA repair protein RadA -
  HS060_RS06815 (HS060_06805) - 1393608..1393838 (-) 231 WP_006251345.1 YgjV family protein -
  HS060_RS06820 (HS060_06810) - 1393957..1394451 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS060_RS06825 (HS060_06815) proB 1394563..1395654 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  HS060_RS06830 (HS060_06820) - 1395654..1396427 (+) 774 WP_409035841.1 TSUP family transporter -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52956.33 Da        Isoelectric Point: 5.3662

>NTDB_id=467988 HS060_RS06800 WP_240020920.1 1390087..1391490(-) (pilB) [Mannheimia haemolytica strain 0152]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGRIGVYQLLNRSAXNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=467988 HS060_RS06800 WP_240020920.1 1390087..1391490(-) (pilB) [Mannheimia haemolytica strain 0152]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGGAAGCGG
TAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCANAAAATTT
TGAAAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATC
AGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.234

98.715

0.713
  pilB Haemophilus influenzae Rd KW20

56.71

98.929

0.561
  pilB Haemophilus influenzae 86-028NP

55.94

99.143

0.555
  pilB Vibrio campbellii strain DS40M4

40.748

100

0.42
  pilB Vibrio cholerae strain A1552

40.249

100

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

38.755

100

0.413
  pilB Legionella pneumophila strain ERS1305867

39.344

100

0.411
  pilB Acinetobacter baylyi ADP1

35.928

100

0.385
  pilB Acinetobacter baumannii D1279779

36.401

100

0.381