Detailed information NTG0463825

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HWQ50_RS27230
• Genome accession: NZ_CP058331
• Coordinates: 5844021..5845724 (+)
• Length: 567 a.a.
• NCBI ID: WP_023100230.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain ACR22
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5826122..5843193	5844021..5845724	flank	828

Gene organization within MGE regions

Location: 5826122..5845724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ50_RS27140 (HWQ50_26975)	-	5826122..5826427 (+)	306	WP_023109345.1	hypothetical protein	-
HWQ50_RS30390 (HWQ50_26980)	-	5826430..5826867 (+)	438	WP_308416434.1	hypothetical protein	-
HWQ50_RS27150 (HWQ50_26985)	-	5826906..5827373 (+)	468	WP_031629313.1	hypothetical protein	-
HWQ50_RS27155 (HWQ50_26990)	-	5827380..5828333 (+)	954	WP_308416433.1	DUF3631 domain-containing protein	-
HWQ50_RS27160 (HWQ50_26995)	-	5828333..5828626 (+)	294	WP_023082016.1	hypothetical protein	-
HWQ50_RS27165 (HWQ50_27000)	-	5828682..5829578 (+)	897	WP_034069047.1	hypothetical protein	-
HWQ50_RS27170 (HWQ50_27005)	-	5830284..5831459 (+)	1176	WP_223822176.1	DUF262 domain-containing protein	-
HWQ50_RS27175 (HWQ50_27010)	-	5831470..5832297 (+)	828	WP_124157035.1	MAE_28990/MAE_18760 family HEPN-like nuclease	-
HWQ50_RS27180	-	5833611..5834117 (-)	507	WP_034064628.1	GNAT family N-acetyltransferase	-
HWQ50_RS27185 (HWQ50_27015)	-	5834890..5835159 (+)	270	WP_225007575.1	hypothetical protein	-
HWQ50_RS27190 (HWQ50_27020)	-	5835159..5835395 (+)	237	WP_003096116.1	hypothetical protein	-
HWQ50_RS27195	-	5835534..5835770 (-)	237	WP_071536299.1	hypothetical protein	-
HWQ50_RS27200 (HWQ50_27025)	-	5835798..5838560 (-)	2763	WP_057379627.1	RHS repeat domain-containing protein	-
HWQ50_RS27205 (HWQ50_27030)	-	5838575..5839723 (-)	1149	WP_172773772.1	DUF6531 domain-containing protein	-
HWQ50_RS27210 (HWQ50_27035)	-	5841902..5842144 (+)	243	WP_023109353.1	hypothetical protein	-
HWQ50_RS27220 (HWQ50_27045)	tfpZ	5842456..5843193 (-)	738	WP_023110409.1	TfpX/TfpZ family type IV pilin accessory protein	-
HWQ50_RS27225	pilE	5843258..5843794 (-)	537	WP_025921208.1	pilin	Machinery gene
HWQ50_RS27230 (HWQ50_27050)	pilB	5844021..5845724 (+)	1704	WP_023100230.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62580.51 Da
• Isoelectric Point: 5.5586

>NTDB_id=463825 HWQ50_RS27230 WP_023100230.1 5844021..5845724(+) (pilB) [Pseudomonas aeruginosa strain ACR22]
MNDTIQLSGLARQLVLHELLDEKAAQQAQLQAQRNKLSLVTYLVQSKLVKGQALTELAADQFGIAYCDLNSLDRDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=463825 HWQ50_RS27230 WP_023100230.1 5844021..5845724(+) (pilB) [Pseudomonas aeruginosa strain ACR22]
ATGAACGATACCATCCAGCTCAGCGGCCTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAAGCAGCTCAACA
GGCCCAGTTGCAAGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGACAGGCCT
TGACCGAACTGGCGGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGATCGGGACAGCCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGGGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGCCTGGATGACGTGGATCTTGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AAGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTAGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGACCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTACGCGCTTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.07	100	0.584
pilB	Acinetobacter baylyi ADP1	57.447	99.471	0.571
pilB	Legionella pneumophila strain ERS1305867	55.282	100	0.554
pilF	Neisseria gonorrhoeae MS11	50.444	99.295	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.763	95.062	0.492
pilB	Vibrio cholerae strain A1552	50.545	97.002	0.49
pilB	Vibrio campbellii strain DS40M4	50.832	95.414	0.485
pilF	Thermus thermophilus HB27	38.584	99.647	0.384
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.189	93.474	0.376