Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HWQ50_RS27225 Genome accession   NZ_CP058331
Coordinates   5843258..5843794 (-) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain ACR22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5826122..5843193 5843258..5843794 flank 65


Gene organization within MGE regions


Location: 5826122..5843794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ50_RS27140 (HWQ50_26975) - 5826122..5826427 (+) 306 WP_023109345.1 hypothetical protein -
  HWQ50_RS30390 (HWQ50_26980) - 5826430..5826867 (+) 438 WP_308416434.1 hypothetical protein -
  HWQ50_RS27150 (HWQ50_26985) - 5826906..5827373 (+) 468 WP_031629313.1 hypothetical protein -
  HWQ50_RS27155 (HWQ50_26990) - 5827380..5828333 (+) 954 WP_308416433.1 DUF3631 domain-containing protein -
  HWQ50_RS27160 (HWQ50_26995) - 5828333..5828626 (+) 294 WP_023082016.1 hypothetical protein -
  HWQ50_RS27165 (HWQ50_27000) - 5828682..5829578 (+) 897 WP_034069047.1 hypothetical protein -
  HWQ50_RS27170 (HWQ50_27005) - 5830284..5831459 (+) 1176 WP_223822176.1 DUF262 domain-containing protein -
  HWQ50_RS27175 (HWQ50_27010) - 5831470..5832297 (+) 828 WP_124157035.1 MAE_28990/MAE_18760 family HEPN-like nuclease -
  HWQ50_RS27180 - 5833611..5834117 (-) 507 WP_034064628.1 GNAT family N-acetyltransferase -
  HWQ50_RS27185 (HWQ50_27015) - 5834890..5835159 (+) 270 WP_225007575.1 hypothetical protein -
  HWQ50_RS27190 (HWQ50_27020) - 5835159..5835395 (+) 237 WP_003096116.1 hypothetical protein -
  HWQ50_RS27195 - 5835534..5835770 (-) 237 WP_071536299.1 hypothetical protein -
  HWQ50_RS27200 (HWQ50_27025) - 5835798..5838560 (-) 2763 WP_057379627.1 RHS repeat domain-containing protein -
  HWQ50_RS27205 (HWQ50_27030) - 5838575..5839723 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  HWQ50_RS27210 (HWQ50_27035) - 5841902..5842144 (+) 243 WP_023109353.1 hypothetical protein -
  HWQ50_RS27220 (HWQ50_27045) tfpZ 5842456..5843193 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  HWQ50_RS27225 pilE 5843258..5843794 (-) 537 WP_025921208.1 pilin Machinery gene

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=463824 HWQ50_RS27225 WP_025921208.1 5843258..5843794(-) (pilE) [Pseudomonas aeruginosa strain ACR22]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=463824 HWQ50_RS27225 WP_025921208.1 5843258..5843794(-) (pilE) [Pseudomonas aeruginosa strain ACR22]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393