Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUT07_RS03735 Genome accession   NZ_CP054931
Coordinates   844879..846606 (+) Length   575 a.a.
NCBI ID   WP_176019793.1    Uniprot ID   A0A7H8NN35
Organism   Stenotrophomonas sp. NA06056     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839879..851606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT07_RS03710 (HUT07_03710) - 840820..841206 (+) 387 WP_176019788.1 hypothetical protein -
  HUT07_RS03715 (HUT07_03715) coaE 841259..841870 (-) 612 WP_176019789.1 dephospho-CoA kinase -
  HUT07_RS03720 (HUT07_03720) - 841882..842745 (-) 864 WP_176019790.1 A24 family peptidase -
  HUT07_RS03725 (HUT07_03725) pilC 842753..844012 (-) 1260 WP_176019791.1 type II secretion system F family protein Machinery gene
  HUT07_RS03730 (HUT07_03730) - 844360..844746 (+) 387 WP_176019792.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HUT07_RS03735 (HUT07_03735) pilB 844879..846606 (+) 1728 WP_176019793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUT07_RS03740 (HUT07_03740) pilR 846736..848109 (-) 1374 WP_176019794.1 sigma-54 dependent transcriptional regulator Regulator
  HUT07_RS03745 (HUT07_03745) - 848164..849777 (-) 1614 WP_176019795.1 ATP-binding protein -
  HUT07_RS03750 (HUT07_03750) sucC 850155..851324 (+) 1170 WP_025874298.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62105.05 Da        Isoelectric Point: 6.1539

>NTDB_id=455388 HUT07_RS03735 WP_176019793.1 844879..846606(+) (pilB) [Stenotrophomonas sp. NA06056]
MNAVTTANLVGITGLARRLVQDGALDEAGARDAMAKATAARQPLPTWFAQNKVVSPPQLAAANAVEFGMPLFDVSVFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTFEQWHSSHDTLGESLGGD
DEALANLDVSGGDEDMAGGDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPNKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKQPADLPAHA
LLAEGFTQAQLDAGIQLHEAVGCDECTGGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=455388 HUT07_RS03735 WP_176019793.1 844879..846606(+) (pilB) [Stenotrophomonas sp. NA06056]
ATGAACGCAGTCACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGACGGCGCACTGGATGA
AGCCGGTGCCCGCGATGCCATGGCCAAGGCCACCGCCGCCCGCCAGCCGCTGCCCACCTGGTTCGCGCAGAACAAGGTGG
TCTCGCCGCCACAGCTGGCCGCAGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCGGTGTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGATGAAGATCAGATCCGTCGCACGTTCGAGCAGTGGCACTCCAGCCACGACACCCTGGGCGAATCGCTCGGCGGCGAT
GATGAAGCGCTGGCCAATCTGGATGTGTCCGGCGGCGACGAGGACATGGCCGGCGGCGATAGCGGCATCGACGCCAAGGG
CGACGATACCCCGGTGGTGAAGTTCATCAACAAGGTGTTGATCGACGCCATCCGCAAGGGCGCCTCGGACATCCACTTCG
AGCCCTACGAAGACGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGAACAAGCTC
AACCAGCGTATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCGGAAAAGCGCGTGCCACAGGATGGCCGCAT
CAAGCTCAACCTCTCCAAGACCAAGCAGATCGATTTCCGCGTCAGCACACTGCCCACGCTGTTCGGCGAGAAGGTGGTGC
TGCGTATCCTTGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACTGGTTCGGGCAAGACGGTGTCGCTGTACACCGC
GCTGGGCATCCTCAATGACGAGACCCGCAACATTTCCACCGCCGAAGATCCGGTGGAAATCCGTCTGCCCGGCGTCAACC
AGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCTGCATTGCGTTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTATCCAC
GCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATTGCCCAGCGTCTGGCGCGCCGCCTGTGTTCGAACTGCAAGCAGCCGGCTGACCTGCCGGCACATGCA
CTGCTGGCGGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGCATCCAGTTGCACGAAGCGGTGGGCTGCGATGAATGCAC
CGGGGGCTACAAGGGCCGTACCGGCCTGTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAATGCGCTGCAGATTGCCGAGGCGGCGCAGGCGATTGGTGTGAACGACCTGCGCCAGTCGGCGTTGAAGAAGGCGGCC
GCGGGTGTAACCAGCCTCGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8NN35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.055

100

0.563
  pilB Acinetobacter baumannii D1279779

56.966

98.609

0.562
  pilB Legionella pneumophila strain ERS1305867

51.839

99.304

0.515
  pilF Neisseria gonorrhoeae MS11

49.648

98.783

0.49
  pilB Vibrio cholerae strain A1552

52.93

89.043

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

46.585

99.304

0.463
  pilB Vibrio campbellii strain DS40M4

45.375

99.652

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.574

95.13

0.367