Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   I9X29_RS25355 Genome accession   NZ_CP065867
Coordinates   5451620..5452084 (-) Length   154 a.a.
NCBI ID   WP_021205141.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain TJ2014-049     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5446620..5457084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9X29_RS25340 (I9X29_25340) nadC 5449131..5449979 (+) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I9X29_RS25350 (I9X29_25350) - 5450159..5451538 (-) 1380 WP_231280141.1 O-antigen ligase family protein -
  I9X29_RS25355 (I9X29_25355) pilA/pilAI 5451620..5452084 (-) 465 WP_021205141.1 pilin Machinery gene
  I9X29_RS25360 (I9X29_25360) pilB 5452315..5454015 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9X29_RS25365 (I9X29_25365) pilC 5454019..5455236 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  I9X29_RS25370 (I9X29_25370) pilD 5455237..5456109 (+) 873 WP_198340918.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  I9X29_RS25375 (I9X29_25375) coaE 5456106..5456717 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  I9X29_RS25380 (I9X29_25380) yacG 5456714..5456914 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16076.44 Da        Isoelectric Point: 9.0145

>NTDB_id=451945 I9X29_RS25355 WP_021205141.1 5451620..5452084(-) (pilA/pilAI) [Pseudomonas aeruginosa strain TJ2014-049]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPANWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=451945 I9X29_RS25355 WP_021205141.1 5451620..5452084(-) (pilA/pilAI) [Pseudomonas aeruginosa strain TJ2014-049]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTGCAGTTGGAAGCCAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTGCTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.945

94.805

0.455
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA Acinetobacter baumannii strain A118

45.205

94.805

0.429
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37