Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ912_RS24385 Genome accession   NZ_CP054128
Coordinates   5482342..5484042 (+) Length   566 a.a.
NCBI ID   WP_047229859.1    Uniprot ID   A0AA96XX08
Organism   Pseudomonas sp. MPDS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5477342..5489042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ912_RS24365 (HQ912_24365) - 5477509..5480442 (+) 2934 WP_047229855.1 DUF748 domain-containing protein -
  HQ912_RS24370 (HQ912_24370) - 5480444..5480740 (+) 297 WP_047229856.1 DUF2845 domain-containing protein -
  HQ912_RS24375 (HQ912_24375) - 5481063..5481416 (-) 354 WP_047229857.1 BON domain-containing protein -
  HQ912_RS24380 (HQ912_24380) pilA 5481697..5482116 (-) 420 WP_047229858.1 pilin Machinery gene
  HQ912_RS24385 (HQ912_24385) pilB 5482342..5484042 (+) 1701 WP_047229859.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HQ912_RS24390 (HQ912_24390) pilC 5484045..5485262 (+) 1218 WP_076386354.1 type II secretion system F family protein Machinery gene
  HQ912_RS24395 (HQ912_24395) pilD 5485264..5486133 (+) 870 WP_047229861.1 prepilin peptidase Machinery gene
  HQ912_RS24400 (HQ912_24400) coaE 5486145..5486768 (+) 624 WP_047229862.1 dephospho-CoA kinase -
  HQ912_RS24405 (HQ912_24405) yacG 5486765..5486965 (+) 201 WP_047229863.1 DNA gyrase inhibitor YacG -
  HQ912_RS24410 (HQ912_24410) - 5486998..5487213 (-) 216 WP_076386357.1 hypothetical protein -
  HQ912_RS24415 (HQ912_24415) - 5487277..5487963 (-) 687 WP_047229865.1 energy-coupling factor ABC transporter permease -
  HQ912_RS24420 (HQ912_24420) - 5488109..5488735 (+) 627 WP_047229866.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62747.81 Da        Isoelectric Point: 5.4229

>NTDB_id=450928 HQ912_RS24385 WP_047229859.1 5482342..5484042(+) (pilB) [Pseudomonas sp. MPDS]
MNDIALSGLAKQLVLAELITEQSAQQAYQQAQSSRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESTSTGLEGMGDVDLDG
LDIESIDDHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKNKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLHNMGIPGFNIATAVHLIIAQRLARKLCPHCKRAIEIPEETLLKEGFPR
ERIGSFTIYEPVGCEQCNNGYKGRVGVYEVVRNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=450928 HQ912_RS24385 WP_047229859.1 5482342..5484042(+) (pilB) [Pseudomonas sp. MPDS]
ATGAATGACATCGCCTTAAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGATCACCGAGCAAAGTGCGCAGCAGGC
GTACCAACAAGCCCAAAGCAGTCGCATGCCCCTGGTCAGTTACCTGGTGCAGAACAAACTGGTCCAGAGTCGCCAGGTGG
CGGAAATTGCCTCGGAGCATTTCGGCGTGGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAACCCAACCCACCGGCCTT
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATTCAGTTCAGCACCGGCTTGACCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAATCCACCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAGTCGATCGATGATCACAAGCAAGACTCCATCGCCGGCCAGGACACCGACGATGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCCGACCTGCACTTTGAACCCTATGAAAAAATCTACCGGG
TGCGGGTGCGTACCGACGGCATGCTGCGAGAGGTGGCCCGCCCGCCGATCCAGTTGGCCACCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTTGATATTTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAAACAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACGCTCTGGGGTGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATGGGTATCGATGCCCTGGGCTACGAGCCGGACCAGAAAGAGCTGTACATGGCGGCGCTCAAACAACCACAAGGG
TTGATTCTGGTGACCGGGCCCACCGGCTCTGGCAAAACCGTGTCGCTCTATACCGGGCTCAATATCCTCAACACTGTGGA
CATCAATATTTCCACCGCTGAGGATCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAGG
GTCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGACACATGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGAC
TGGCGCGCAAGCTGTGCCCGCATTGCAAAAGGGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTTCCGCGG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGCTACAAGGGGCGCGTGGGGGT
TTACGAAGTGGTCAGGAATACGCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGTTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTTAATGACCTGCGCGCCTCGGGACTGCTCAAAGTGATGCAAGGCATCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

56.738

99.647

0.565
  pilB Legionella pneumophila strain ERS1305867

55.458

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilF Neisseria gonorrhoeae MS11

48.668

99.47

0.484
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.088

96.82

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.833

99.293

0.366