Detailed information NTG0449665

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FOC87_RS10370
• Genome accession: NZ_CP053981
• Coordinates: 2027548..2031273 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000572292.1
• Uniprot ID: A0RAX7
• Organism: Bacillus thuringiensis strain FDAARGOS_793
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2022548..2036273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC87_RS10355 (FOC87_10355)	-	2022706..2023299 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
FOC87_RS10360 (FOC87_10360)	lepB	2023356..2023919 (+)	564	WP_000751894.1	signal peptidase I	-
FOC87_RS10365 (FOC87_10365)	addB	2024036..2027551 (+)	3516	WP_000058569.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FOC87_RS10370 (FOC87_10370)	addA	2027548..2031273 (+)	3726	WP_000572292.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FOC87_RS10375 (FOC87_10375)	-	2031286..2031573 (+)	288	WP_000718623.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FOC87_RS10380 (FOC87_10380)	gerPF	2031611..2031826 (-)	216	WP_001141566.1	spore germination protein GerPF	-
FOC87_RS10385 (FOC87_10385)	-	2031869..2032255 (-)	387	WP_000902341.1	spore germination protein GerPE	-
FOC87_RS10390 (FOC87_10390)	gerPD	2032271..2032465 (-)	195	WP_001052802.1	spore germination protein GerPD	-
FOC87_RS10395 (FOC87_10395)	gerPC	2032472..2033086 (-)	615	WP_001070766.1	spore germination protein GerPC	-
FOC87_RS10400 (FOC87_10400)	gerPB	2033154..2033360 (-)	207	WP_001012512.1	spore germination protein GerPB	-
FOC87_RS10405 (FOC87_10405)	gerPA	2033375..2033596 (-)	222	WP_001111188.1	spore germination protein GerPA	-
FOC87_RS10410 (FOC87_10410)	-	2033693..2033872 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FOC87_RS10415 (FOC87_10415)	-	2034120..2035019 (+)	900	WP_001241732.1	fumarylacetoacetate hydrolase family protein	-
FOC87_RS10420 (FOC87_10420)	-	2035055..2035339 (-)	285	WP_000925338.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142662.93 Da
• Isoelectric Point: 4.8339

>NTDB_id=449665 FOC87_RS10370 WP_000572292.1 2027548..2031273(+) (addA) [Bacillus thuringiensis strain FDAARGOS_793]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
ETRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=449665 FOC87_RS10370 WP_000572292.1 2027548..2031273(+) (addA) [Bacillus thuringiensis strain FDAARGOS_793]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCGGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACGCAAGACTTAATGAA
ACGTTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAACTCGTTACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

3D structure

• AlphaFold DB: A0RAX7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.328	100	0.536