Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   G4D73_RS11105 Genome accession   NZ_CP053947
Coordinates   2387689..2388747 (+) Length   352 a.a.
NCBI ID   WP_004644949.1    Uniprot ID   N9Q4M1
Organism   Acinetobacter sp. WY4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382689..2393747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4D73_RS11090 mraY 2382971..2384089 (+) 1119 WP_213686771.1 phospho-N-acetylmuramoyl-pentapeptide- transferase -
  G4D73_RS11095 - 2384144..2384947 (+) 804 WP_213687747.1 putative RNA methyltransferase -
  G4D73_RS11100 ponA 2384998..2387523 (-) 2526 WP_213686772.1 penicillin-binding protein PBP1a -
  G4D73_RS11105 pilM 2387689..2388747 (+) 1059 WP_004644949.1 pilus assembly protein PilM Machinery gene
  G4D73_RS11110 comN 2388747..2389385 (+) 639 WP_004281141.1 PilN domain-containing protein Machinery gene
  G4D73_RS11115 comO 2389382..2390104 (+) 723 WP_213686773.1 type 4a pilus biogenesis protein PilO Machinery gene
  G4D73_RS11120 pilP 2390104..2390631 (+) 528 WP_004732447.1 pilus assembly protein PilP Machinery gene
  G4D73_RS11125 pilQ 2390694..2392829 (+) 2136 WP_416232266.1 type IV pilus secretin PilQ Machinery gene
  G4D73_RS11130 aroK 2392866..2393402 (+) 537 WP_213686775.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39037.53 Da        Isoelectric Point: 4.5358

>NTDB_id=449309 G4D73_RS11105 WP_004644949.1 2387689..2388747(+) (pilM) [Acinetobacter sp. WY4]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSGRYWVESYALIPLSENSVVEKNILNPEAVADALGRAINLGNTQSNQAAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLSNPNRVNVLLVATRIENVEARSEVLEI
SSLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=449309 G4D73_RS11105 WP_004644949.1 2387689..2388747(+) (pilM) [Acinetobacter sp. WY4]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAGGTTTTAGAACT
TTCTGTAAAAAGTGGCCGTTACTGGGTAGAAAGCTATGCTTTGATCCCATTATCGGAAAACAGTGTTGTTGAAAAAAACA
TCTTAAACCCAGAAGCAGTCGCTGATGCCCTTGGGCGTGCAATTAATTTGGGCAATACCCAGTCGAATCAGGCCGCTTTT
GCGATTCCAACTTCAATGGTCATCACCAAAATCATTGAGATGGATGCAGACATGTCAGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCAGAACAATACATTCCTTTTCCACTCGATGAAGCAAGTCTGGATTTTGAAGTTCTGCCAGACCGCCTTT
CCAATCCGAACCGGGTCAATGTACTGCTGGTGGCGACACGAATCGAAAATGTCGAAGCACGTTCTGAAGTTTTAGAGATT
AGCAGTCTAACGCCTAAGATTGCAGATGTAGAAAGTTTTGCGCTGGAAAATGCCTTTAAGGTATTTTCGGATACTTTACC
GATGGGGGTCAATACGGTTGGTATTTTAGACATCGGTCATAGTATGACGACCTTGTCGGTGATGCAGAACAACAAGATTA
TTTATACCCGTGAGCAGGTGTTTGGCGGCAAGCAATTGACTCAGGAAATTCAGAATCGTTATGGATTATCCTTTGAAGAA
GCTGGACGTGCTAAAAAAACCCGTGCCTTGCCTGATGATTATGATATTGAAGTCCTAGAACCCTTCTTGGATGCCGTGGT
ACAACAGGCAGCCCGTTCATTACAATTCTTCTTTTCATCTTCTCAGTTTAATGAGATTGACCATATTTTACTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCGCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGCGGTTTGGC
ATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9Q4M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

85.227

100

0.852
  comM Acinetobacter nosocomialis M2

85.227

100

0.852
  comM Acinetobacter baylyi ADP1

79.261

100

0.793
  pilM Legionella pneumophila strain ERS1305867

39.266

100

0.395