Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BMWSH_RS06915 Genome accession   NC_017138
Coordinates   1379581..1380021 (-) Length   146 a.a.
NCBI ID   WP_014458694.1    Uniprot ID   A0A8D3WZE0
Organism   Priestia megaterium WSH-002     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1374581..1385021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMWSH_RS06895 (BMWSH_1343) - 1374582..1375130 (+) 549 WP_014458689.1 enoyl-CoA hydratase/isomerase family protein -
  BMWSH_RS27280 (BMWSH_1344) - 1375239..1375364 (+) 126 WP_014458690.1 hypothetical protein -
  BMWSH_RS06900 (BMWSH_1345) helD 1375608..1377926 (+) 2319 WP_014458691.1 RNA polymerase recycling motor HelD -
  BMWSH_RS06905 (BMWSH_1346) - 1378315..1378905 (+) 591 WP_014458692.1 CPBP family intramembrane glutamic endopeptidase -
  BMWSH_RS06910 (BMWSH_1347) comJ 1379138..1379545 (-) 408 WP_014458693.1 competence protein ComJ -
  BMWSH_RS06915 (BMWSH_1348) nucA/comI 1379581..1380021 (-) 441 WP_014458694.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BMWSH_RS06920 (BMWSH_1349) - 1380399..1380659 (+) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  BMWSH_RS06925 (BMWSH_1350) - 1380816..1382765 (+) 1950 WP_014458696.1 FUSC family protein -
  BMWSH_RS06930 (BMWSH_1351) - 1382801..1383619 (-) 819 WP_014458697.1 squalene/phytoene synthase family protein -
  BMWSH_RS06935 (BMWSH_1352) - 1383945..1384616 (+) 672 WP_014458698.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16004.83 Da        Isoelectric Point: 4.4411

>NTDB_id=44867 BMWSH_RS06915 WP_014458694.1 1379581..1380021(-) (nucA/comI) [Priestia megaterium WSH-002]
MSTYGKKLMASLFIIIACVLGFIGHAAFLAPEKTEAEANYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEAN
REESLKGYPTKTGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=44867 BMWSH_RS06915 WP_014458694.1 1379581..1380021(-) (nucA/comI) [Priestia megaterium WSH-002]
ATGAGTACATACGGGAAAAAATTAATGGCAAGTCTATTCATTATCATTGCATGCGTTTTAGGGTTTATAGGACATGCGGC
GTTTTTAGCTCCTGAAAAAACGGAAGCCGAAGCAAACTATGATGCAACGCTTTATTTTCCACTGGACCGATATCCTGAAA
CCGGCGATCATATTCGCGACGCAATTGCTGCTGGTCACTCTTCCGTTTGCACAATTGATCGCGATGGTGCTGAAGCAAAC
CGAGAAGAATCGCTAAAAGGATACCCAACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCTATGTGCGAAGAAGG
CGGAGCAGGAGCTGATATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGGAAATCAGTTAGAAC
AGTACCCAGATGGTACACGCGTACAATTTATCGTGCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

65.079

86.301

0.562

Multiple sequence alignment