Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BMWSH_RS09575 Genome accession   NC_017138
Coordinates   1854211..1854633 (-) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium WSH-002     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1849211..1859633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMWSH_RS09545 (BMWSH_1926) - 1849509..1849994 (+) 486 WP_014459237.1 hypothetical protein -
  BMWSH_RS09550 (BMWSH_1927) - 1850126..1850671 (-) 546 WP_014459238.1 GNAT family N-acetyltransferase -
  BMWSH_RS09555 (BMWSH_1928) - 1850971..1852248 (+) 1278 WP_014459239.1 glycosyltransferase -
  BMWSH_RS09560 (BMWSH_1929) - 1852272..1852928 (+) 657 WP_014459240.1 TetR/AcrR family transcriptional regulator -
  BMWSH_RS09565 (BMWSH_1930) - 1853082..1853660 (+) 579 WP_014459241.1 hypothetical protein -
  BMWSH_RS09570 (BMWSH_1931) - 1853717..1854061 (-) 345 WP_014459242.1 DUF1516 family protein -
  BMWSH_RS09575 (BMWSH_1932) nucA/comI 1854211..1854633 (-) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BMWSH_RS09580 (BMWSH_1933) - 1854774..1855238 (-) 465 WP_014459243.1 FixH family protein -
  BMWSH_RS09585 (BMWSH_1934) - 1855268..1856650 (-) 1383 WP_014459244.1 PepSY domain-containing protein -
  BMWSH_RS09590 (BMWSH_1935) - 1856788..1857429 (-) 642 WP_014459245.1 response regulator transcription factor -
  BMWSH_RS09595 (BMWSH_1936) - 1857430..1858251 (-) 822 WP_014459246.1 sensor histidine kinase -
  BMWSH_RS09600 (BMWSH_1937) - 1858472..1859305 (+) 834 WP_014459247.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=44870 BMWSH_RS09575 WP_013057949.1 1854211..1854633(-) (nucA/comI) [Priestia megaterium WSH-002]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=44870 BMWSH_RS09575 WP_013057949.1 1854211..1854633(-) (nucA/comI) [Priestia megaterium WSH-002]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGTATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGTTCTTGGATTTCACATCAAGTAGATGAGTTTCCAGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507

Multiple sequence alignment