Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HQN78_RS23020 Genome accession   NZ_CP053750
Coordinates   4930418..4932124 (+) Length   568 a.a.
NCBI ID   WP_107800583.1    Uniprot ID   A0A2T5NM57
Organism   Chromobacterium sp. Beijing     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4925418..4937124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN78_RS23000 (HQN78_22770) - 4926032..4926853 (+) 822 WP_107800587.1 inner membrane protein YpjD -
  HQN78_RS23005 (HQN78_22775) - 4926887..4927264 (+) 378 WP_235086593.1 helix-hairpin-helix domain-containing protein -
  HQN78_RS23010 (HQN78_22780) - 4927318..4928718 (-) 1401 WP_235086595.1 deoxyribodipyrimidine photo-lyase -
  HQN78_RS23015 (HQN78_22785) - 4929068..4930198 (+) 1131 WP_158274338.1 porin -
  HQN78_RS23020 (HQN78_22790) pilF 4930418..4932124 (+) 1707 WP_107800583.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HQN78_RS23025 (HQN78_22795) pilC 4932196..4933419 (+) 1224 WP_107800582.1 type II secretion system F family protein Machinery gene
  HQN78_RS23030 (HQN78_22800) - 4933416..4934284 (+) 869 Protein_4533 A24 family peptidase -
  HQN78_RS23035 (HQN78_22805) coaE 4934298..4934885 (+) 588 Protein_4534 dephospho-CoA kinase -
  HQN78_RS23040 (HQN78_22810) zapD 4934924..4935682 (+) 759 WP_019100891.1 cell division protein ZapD -
  HQN78_RS23045 (HQN78_22815) yacG 4935722..4935910 (+) 189 WP_048409544.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62124.71 Da        Isoelectric Point: 5.5900

>NTDB_id=446924 HQN78_RS23020 WP_107800583.1 4930418..4932124(+) (pilF) [Chromobacterium sp. Beijing]
MEASAGISGLGRALVQNNMLLQQDADAILRQSQASHISFVEQLIQSKKMTAQDVAMFASKTFGYPLLDMSAIDLDQLPKG
VVDDELIASRRLLPLLKRGNKLYVGTSDPTQTSAYHAITFKTGMTVEVVVVEDDKLGKMLGKYGEDATAAIKALENEDFG
DLEFADPDAPADTGAQPDVDDAPVVKFIHKVLLDAINGGASDIHFEPYEKFYRIRYRVDGVLKEISQPPLVLKEKIASRI
KVISKLDISEKRVPQDGRMKLVISKARAIDFRVSTLPTLYGEKIVMRILDSSAANLDIEQLGFEPEQKRLLLDAIGRPYG
MVLVTGPTGSGKTVSLYTCLNLLNKPDINIATAEDPVEINLPGINQVNVNERAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARRLCAHCKKPADIPEEALLRAGFKK
EELDGSWKPYAPVGCEECRNTGYRGRTGIYEVMPITDAMTRLIMQNGTAIDIADLARKEGMVDLRHAGLLKVKAGLTSLT
EIEAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=446924 HQN78_RS23020 WP_107800583.1 4930418..4932124(+) (pilF) [Chromobacterium sp. Beijing]
ATGGAAGCTTCGGCTGGCATTTCCGGCTTGGGTCGGGCCTTGGTGCAAAACAATATGCTGTTGCAGCAGGATGCGGACGC
CATTCTGCGTCAGTCTCAGGCATCGCATATCAGCTTTGTCGAGCAGCTGATTCAGAGCAAGAAGATGACGGCGCAGGATG
TCGCGATGTTCGCGTCCAAGACCTTTGGCTATCCCTTATTGGACATGTCGGCCATCGATCTCGATCAGTTGCCCAAGGGC
GTGGTGGATGACGAGCTGATCGCCAGCCGGCGCTTGCTGCCCCTGCTCAAGCGCGGCAACAAGCTGTATGTCGGCACCTC
GGACCCGACTCAGACCTCGGCCTATCACGCCATCACCTTCAAGACCGGGATGACGGTGGAAGTGGTGGTGGTGGAGGACG
ACAAGCTGGGCAAGATGCTCGGCAAGTACGGCGAGGACGCCACCGCCGCGATCAAGGCCCTGGAAAACGAGGATTTTGGC
GATCTCGAATTCGCCGATCCGGACGCGCCGGCGGATACCGGCGCGCAGCCGGACGTCGACGACGCGCCGGTGGTCAAATT
CATCCACAAGGTGTTGCTGGACGCGATCAATGGCGGCGCGTCCGATATCCATTTCGAACCCTATGAGAAGTTCTACCGCA
TTCGTTACCGGGTGGACGGGGTGTTGAAGGAGATCTCGCAGCCGCCCTTGGTGTTGAAGGAAAAGATCGCCTCCCGGATC
AAGGTGATTTCCAAGCTGGATATTTCGGAAAAGCGGGTGCCGCAGGATGGCCGGATGAAGCTGGTCATTTCCAAGGCGCG
GGCGATCGATTTCCGGGTCAGCACCTTGCCCACTTTGTACGGCGAAAAGATCGTGATGCGTATCCTGGACTCGTCGGCGG
CGAATCTGGATATCGAGCAGCTCGGCTTCGAGCCGGAGCAGAAACGTTTGTTGCTGGACGCGATCGGGCGCCCCTACGGC
ATGGTGCTGGTGACCGGCCCCACCGGCAGCGGTAAAACGGTGTCGCTGTATACCTGTCTTAATTTGCTGAACAAGCCGGA
CATCAATATCGCCACGGCCGAGGACCCGGTGGAAATCAACCTGCCGGGCATCAATCAGGTCAACGTCAACGAGCGCGCCG
GGCTGACCTTCGCCTCGGCCCTGAAGGCTTTTCTGCGCCAGGATCCGGACGTGATCATGGTGGGCGAGATCCGGGATTTC
GAAACGGCGGACATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTTCTCCACCCTGCATACCAATAACGCGCCGGC
GACTTTGACCCGGATGCTGAACATGGGGGTGGCGCCGTTCAATATCGCCAGCTCGGTGCTGCTGATCATGGCGCAGCGCC
TGGCGCGCCGTTTGTGCGCGCATTGCAAGAAGCCGGCGGACATTCCCGAAGAGGCGCTGTTGCGGGCGGGTTTCAAGAAA
GAGGAATTGGACGGCAGCTGGAAGCCCTATGCGCCGGTGGGCTGTGAGGAGTGCCGCAATACCGGCTACCGCGGCCGTAC
CGGCATTTACGAAGTGATGCCCATCACCGACGCGATGACGCGTTTGATCATGCAGAACGGCACCGCCATCGATATCGCCG
ATCTGGCGCGCAAGGAGGGCATGGTGGATTTGCGCCACGCCGGCCTGCTGAAGGTCAAGGCGGGCTTGACCTCGTTGACC
GAGATCGAGGCGGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T5NM57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.283

99.472

0.56
  pilB Acinetobacter baumannii D1279779

53.886

100

0.549
  pilB Acinetobacter baylyi ADP1

53.819

99.12

0.533
  pilB Legionella pneumophila strain ERS1305867

52.297

99.648

0.521
  pilB Vibrio cholerae strain A1552

49.91

98.063

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

46.797

98.944

0.463
  pilB Vibrio campbellii strain DS40M4

45.826

99.12

0.454
  pilF Thermus thermophilus HB27

38.23

99.472

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.79

98.768

0.373