Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I3251_RS13915 Genome accession   NZ_CP065374
Coordinates   2959012..2960076 (-) Length   354 a.a.
NCBI ID   WP_122329547.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PAG7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954012..2965076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3251_RS13890 (I3251_13890) aroK 2954547..2955065 (-) 519 WP_003095833.1 shikimate kinase AroK -
  I3251_RS13895 (I3251_13895) pilQ 2955077..2957221 (-) 2145 Protein_2747 type 4a pilus secretin PilQ -
  I3251_RS13900 (I3251_13900) comL/pilP 2957275..2957799 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  I3251_RS13905 (I3251_13905) pilO 2957796..2958419 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3251_RS13910 (I3251_13910) pilN 2958416..2959012 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  I3251_RS13915 (I3251_13915) pilM 2959012..2960076 (-) 1065 WP_122329547.1 type IV pilus assembly protein PilM Machinery gene
  I3251_RS13920 (I3251_13920) - 2960261..2962732 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -
  I3251_RS13925 (I3251_13925) - 2962837..2964105 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37982.54 Da        Isoelectric Point: 4.6380

>NTDB_id=446532 I3251_RS13915 WP_122329547.1 2959012..2960076(-) (pilM) [Pseudomonas aeruginosa strain PAG7]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEKVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=446532 I3251_RS13915 WP_122329547.1 2959012..2960076(-) (pilM) [Pseudomonas aeruginosa strain PAG7]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGAAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM/pilM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463