Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   I3251_RS13910 Genome accession   NZ_CP065374
Coordinates   2958416..2959012 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain PAG7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2953416..2964012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3251_RS13890 (I3251_13890) aroK 2954547..2955065 (-) 519 WP_003095833.1 shikimate kinase AroK -
  I3251_RS13895 (I3251_13895) pilQ 2955077..2957221 (-) 2145 Protein_2747 type 4a pilus secretin PilQ -
  I3251_RS13900 (I3251_13900) comL/pilP 2957275..2957799 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  I3251_RS13905 (I3251_13905) pilO 2957796..2958419 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3251_RS13910 (I3251_13910) pilN 2958416..2959012 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  I3251_RS13915 (I3251_13915) pilM 2959012..2960076 (-) 1065 WP_122329547.1 type IV pilus assembly protein PilM Machinery gene
  I3251_RS13920 (I3251_13920) - 2960261..2962732 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=446531 I3251_RS13910 WP_003095839.1 2958416..2959012(-) (pilN) [Pseudomonas aeruginosa strain PAG7]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=446531 I3251_RS13910 WP_003095839.1 2958416..2959012(-) (pilN) [Pseudomonas aeruginosa strain PAG7]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369