Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I3251_RS13905 Genome accession   NZ_CP065374
Coordinates   2957796..2958419 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PAG7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2952796..2963419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3251_RS13885 (I3251_13885) aroB 2953391..2954497 (-) 1107 WP_003116138.1 3-dehydroquinate synthase -
  I3251_RS13890 (I3251_13890) aroK 2954547..2955065 (-) 519 WP_003095833.1 shikimate kinase AroK -
  I3251_RS13895 (I3251_13895) pilQ 2955077..2957221 (-) 2145 Protein_2747 type 4a pilus secretin PilQ -
  I3251_RS13900 (I3251_13900) comL/pilP 2957275..2957799 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  I3251_RS13905 (I3251_13905) pilO 2957796..2958419 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  I3251_RS13910 (I3251_13910) pilN 2958416..2959012 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  I3251_RS13915 (I3251_13915) pilM 2959012..2960076 (-) 1065 WP_122329547.1 type IV pilus assembly protein PilM Machinery gene
  I3251_RS13920 (I3251_13920) - 2960261..2962732 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=446530 I3251_RS13905 WP_003103268.1 2957796..2958419(-) (pilO) [Pseudomonas aeruginosa strain PAG7]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=446530 I3251_RS13905 WP_003103268.1 2957796..2958419(-) (pilO) [Pseudomonas aeruginosa strain PAG7]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTT
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401