Detailed information NTG0446215

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: HPC60_RS12200
• Genome accession: NZ_CP053671
• Coordinates: 2391491..2391937 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis subsp. lactis strain G121
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2386491..2396937

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPC60_RS12165 (HPC60_06915)	-	2386600..2386977 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
HPC60_RS12170 (HPC60_06920)	-	2387182..2388048 (+)	867	WP_025017140.1	RluA family pseudouridine synthase	-
HPC60_RS12175 (HPC60_06925)	-	2388086..2388895 (-)	810	WP_014570791.1	metal ABC transporter permease	-
HPC60_RS12180 (HPC60_06930)	-	2388888..2389625 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
HPC60_RS12185 (HPC60_06935)	-	2389802..2390644 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
HPC60_RS12190 (HPC60_06940)	-	2390641..2391078 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
HPC60_RS12195 (HPC60_06945)	comGG	2391168..2391452 (-)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
HPC60_RS12200 (HPC60_06950)	comGF	2391491..2391937 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
HPC60_RS12205 (HPC60_06955)	comGE	2391900..2392196 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
HPC60_RS12210 (HPC60_06960)	comGD	2392168..2392584 (-)	417	WP_025017137.1	competence type IV pilus minor pilin ComGD	Machinery gene
HPC60_RS12215 (HPC60_06965)	comGC	2392559..2392882 (-)	324	WP_032943614.1	competence type IV pilus major pilin ComGC	Machinery gene
HPC60_RS12220 (HPC60_06970)	comGB	2392956..2394029 (-)	1074	WP_074453836.1	competence type IV pilus assembly protein ComGB	Machinery gene
HPC60_RS12225 (HPC60_06975)	comGA	2393923..2394861 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=446215 HPC60_RS12200 WP_029344525.1 2391491..2391937(-) (comGF) [Lactococcus lactis subsp. lactis strain G121]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=446215 HPC60_RS12200 WP_029344525.1 2391491..2391937(-) (comGF) [Lactococcus lactis subsp. lactis strain G121]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385