Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HND92_RS05190 Genome accession   NZ_CP053553
Coordinates   1049853..1051586 (-) Length   577 a.a.
NCBI ID   WP_012655751.1    Uniprot ID   A0A6M6IAK8
Organism   Diaphorobacter sp. JS3050     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1044853..1056586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HND92_RS05160 (HND92_05165) - 1045511..1045957 (+) 447 WP_011804248.1 NUDIX domain-containing protein -
  HND92_RS05165 (HND92_05170) - 1046026..1046256 (-) 231 WP_011804249.1 DNA gyrase inhibitor YacG -
  HND92_RS05170 (HND92_05175) zapD 1046263..1047018 (-) 756 WP_172206340.1 cell division protein ZapD -
  HND92_RS05175 (HND92_05180) coaE 1047109..1047702 (-) 594 WP_253940107.1 dephospho-CoA kinase -
  HND92_RS05180 (HND92_05185) - 1047734..1048615 (-) 882 WP_172206345.1 A24 family peptidase -
  HND92_RS05185 (HND92_05190) pilC 1048615..1049832 (-) 1218 WP_012655750.1 type II secretion system F family protein Machinery gene
  HND92_RS05190 (HND92_05195) pilB 1049853..1051586 (-) 1734 WP_012655751.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HND92_RS05200 (HND92_05205) - 1051927..1052856 (-) 930 WP_047350041.1 polyprenyl synthetase family protein -
  HND92_RS05205 (HND92_05210) rplU 1053081..1053392 (+) 312 WP_011804285.1 50S ribosomal protein L21 -
  HND92_RS05210 (HND92_05215) rpmA 1053410..1053667 (+) 258 WP_011804286.1 50S ribosomal protein L27 -
  HND92_RS05215 (HND92_05220) cgtA 1053814..1054887 (+) 1074 WP_172206347.1 Obg family GTPase CgtA -
  HND92_RS05220 (HND92_05225) proB 1054961..1056103 (+) 1143 WP_047349967.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63629.02 Da        Isoelectric Point: 5.3559

>NTDB_id=445100 HND92_RS05190 WP_012655751.1 1049853..1051586(-) (pilB) [Diaphorobacter sp. JS3050]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIIAEYDKLLRLIEASTKSGTETLD
GINSSNDFEFGDIPVDEAPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=445100 HND92_RS05190 WP_012655751.1 1049853..1051586(-) (pilB) [Diaphorobacter sp. JS3050]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATTGCCTTACCCGGACTGGCGCGCGCGTTGATGTCTGTGGGCAA
ATTGGATCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTGGCCCATACGGTATCCACCGTATTTGGTGCCCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGATCTGTTGGACGTCAAACTGTGCCAGTCCTACAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATCGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCGCGGAATATGACAAGCTGCTCCGCTTGATTGAGGCGAGCACGAAGAGCGGTACAGAAACGCTCGAC
GGCATCAACAGCAGTAATGATTTTGAGTTCGGCGATATTCCCGTTGATGAAGCTCCGGACGAGGATGCCGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGCTGGATGCTTTCAATATGCGTGCATCCGATCTGC
ATTTTGAGCCCTATGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGTGAAATCGCATCGCCGCCTATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGT
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCCTGGTGACCGGACCCACGGGCTCCGGGAAGACCGTGTCGCTCTA
CACATGTCTCAATTTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGACCCCTCGGAAATCAATCTCCCGGGCG
TGAACCAAGTCAACGTCAATGAGAAAGCCGGCCTCACGTTTGCCGTCGCGCTCAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACGGCAGACATTTCAATCAAAGCCGCGCAGACGGGTCACTTGGTGCT
CTCTACCCTGCACACCAATGACGCTCCCACGACATTGACGCGCATGCGCAATATGGGCATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCGCAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTTACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCGCTGGAAATTGCCGAACAGGCACGCCTGGAGGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGATTGACCTCCTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6IAK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.181

99.48

0.539
  pilB Acinetobacter baumannii D1279779

53.723

97.747

0.525
  pilF Neisseria gonorrhoeae MS11

52.389

97.92

0.513
  pilB Legionella pneumophila strain ERS1305867

49.912

97.92

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

46.881

91.681

0.43
  pilF Thermus thermophilus HB27

39.897

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

92.721

0.381