Detailed information NTG0444697

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HLV59_RS14215
• Genome accession: NZ_CP053391
• Coordinates: 3043352..3044470 (+)
• Length: 372 a.a.
• NCBI ID: WP_126646067.1
• Uniprot ID: -
• Organism: Acinetobacter lactucae isolate QL-1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3038352..3049470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HLV59_RS14205	-	3041476..3042168 (-)	693	WP_171430238.1	YggS family pyridoxal phosphate-dependent enzyme	-
HLV59_RS14210	pilT	3042295..3043332 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
HLV59_RS14215	pilU	3043352..3044470 (+)	1119	WP_126646067.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HLV59_RS14220	fur	3044540..3044977 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
HLV59_RS14225	-	3045089..3045487 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
HLV59_RS14230	-	3045552..3045866 (-)	315	WP_016144536.1	RnfH family protein	-
HLV59_RS14235	-	3045866..3046933 (-)	1068	WP_099910029.1	hypothetical protein	-
HLV59_RS14240	-	3047087..3047542 (-)	456	WP_002121698.1	bacteriohemerythrin	-
HLV59_RS14245	-	3047794..3048642 (-)	849	WP_016144533.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6401

>NTDB_id=444697 HLV59_RS14215 WP_126646067.1 3043352..3044470(+) (pilU) [Acinetobacter lactucae isolate QL-1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=444697 HLV59_RS14215 WP_126646067.1 3043352..3044470(+) (pilU) [Acinetobacter lactucae isolate QL-1]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCGTC
GATGAAGATTAATGGACAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTCCGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GCACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGTAATCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCAATCGAGTTTATTCACGAACATGCAGGCTGCATCATTACCCAACGTGAAGTCGGAATCGATACGGACTCATTTGA
AATTGCCTTAAAAAATACTTTGCGACAAGCACCCGATGTCATCTTGATTGGTGAGATTCGTACGCGTGAAGTGATGGACT
ATGCAATTGGCTTCGCCGAAACGGGTCACTTGGTCTTAGCCACAATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCATTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACCTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (40-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36