Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HNO53_RS09195 Genome accession   NZ_CP053383
Coordinates   1973507..1975300 (-) Length   597 a.a.
NCBI ID   WP_242597592.1    Uniprot ID   -
Organism   Halomonas sulfidivorans strain MCCC 1A13718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1968507..1980300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNO53_RS09165 (HNO53_09165) - 1968809..1969552 (+) 744 WP_209478020.1 tellurite resistance TerB family protein -
  HNO53_RS09170 (HNO53_09170) - 1969582..1970445 (-) 864 WP_209478587.1 DNA replication terminus site-binding protein -
  HNO53_RS09175 (HNO53_09175) yacG 1970448..1970675 (-) 228 WP_209478022.1 DNA gyrase inhibitor YacG -
  HNO53_RS09180 (HNO53_09180) coaE 1970709..1971314 (-) 606 WP_209478024.1 dephospho-CoA kinase -
  HNO53_RS09185 (HNO53_09185) pilD 1971334..1972164 (-) 831 WP_242597591.1 prepilin peptidase Machinery gene
  HNO53_RS09190 (HNO53_09190) pilC 1972161..1973393 (-) 1233 WP_209478026.1 type II secretion system F family protein Machinery gene
  HNO53_RS09195 (HNO53_09195) pilB 1973507..1975300 (-) 1794 WP_242597592.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNO53_RS09200 - 1975725..1975856 (+) 132 Protein_1811 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HNO53_RS09205 (HNO53_09205) - 1976249..1978039 (+) 1791 WP_209478028.1 DUF7305 domain-containing protein -
  HNO53_RS09210 (HNO53_09210) - 1978122..1978640 (+) 519 WP_209478030.1 anti-virulence regulator CigR family protein -
  HNO53_RS09215 (HNO53_09215) - 1978713..1979576 (-) 864 WP_209478031.1 glycine betaine ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65422.81 Da        Isoelectric Point: 5.5424

>NTDB_id=444655 HNO53_RS09195 WP_242597592.1 1973507..1975300(-) (pilB) [Halomonas sulfidivorans strain MCCC 1A13718]
MNDYRTAPAGAQETASHSANPPRAADTEGLRGFARRLVEYGLLSRAAAERAEHEAREAETSLLQHVIDAGLVTARQGTLC
AAWEYGLPLVDLDALRLSSLPPANEYPEKLLRKLCVVPLSRRSHRLTVAVPYPSTLALLDELQFATGLSIEAVLCPVDQL
MPVLEAYLAQHESSMMDELAGVDDAISELEFDDGVDISEERASDAAQSGADDAPIVKFVNKILLDAIRRGASDIHFEPYE
TSYRVRMRVDGMLMESARPPFAMRSRIAARLKVMARLDISERRLPQDGAIKLKVSKTRSIDFRVNSLPTVYGEKIVLRIL
DPTSAQIGIDALGFTPDQRTLYEGVLDHPQGMILVTGPTGSGKTVTLYTGLNILNEVQRNICTAEDPVEIKVPGVNQVNV
LPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVPAFNIASSVSLI
IAQRLARRLCKHCRQEAELPREVLLQEGFTEQEVDSATVYEAVGCKHCTHGYKGRVGIYEVVPISNAMSQLIMRNGNSMD
FDSLARQEGHPDLRRSGLLKVMQGITSLTEINRVTKD

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=444655 HNO53_RS09195 WP_242597592.1 1973507..1975300(-) (pilB) [Halomonas sulfidivorans strain MCCC 1A13718]
ATGAACGACTATCGAACGGCACCGGCCGGTGCCCAGGAGACAGCCAGCCACAGCGCCAACCCACCGCGAGCGGCCGACAC
CGAGGGGCTGCGCGGTTTCGCTCGTCGGCTGGTGGAGTACGGGCTGCTTTCCCGCGCGGCCGCCGAGCGGGCGGAACATG
AGGCCCGGGAGGCCGAGACGAGCCTGCTCCAGCATGTGATCGATGCCGGCCTGGTCACGGCGCGCCAGGGCACGCTGTGT
GCGGCATGGGAGTACGGCCTGCCACTGGTGGATCTCGACGCGCTGCGCCTGTCGAGCCTGCCGCCGGCCAACGAATACCC
CGAGAAGCTGCTGCGCAAGCTGTGTGTGGTGCCGCTCAGTCGCCGCAGCCACCGCTTGACCGTTGCGGTGCCCTACCCCT
CCACACTGGCCCTGCTCGACGAGCTGCAATTCGCTACCGGCCTGAGCATCGAGGCCGTGCTCTGCCCGGTGGATCAGTTG
ATGCCGGTGCTCGAGGCCTACTTGGCGCAGCATGAGTCGAGCATGATGGACGAGCTGGCGGGCGTGGATGATGCCATAAG
CGAACTGGAATTCGACGATGGCGTCGACATCAGCGAGGAACGCGCCAGCGATGCTGCCCAGAGCGGGGCCGATGACGCGC
CGATCGTCAAGTTCGTCAACAAGATCCTGCTCGATGCCATTCGCCGCGGGGCGTCGGATATCCACTTCGAACCCTACGAG
ACCAGCTATCGGGTGCGCATGCGCGTGGACGGCATGCTAATGGAGTCGGCGCGGCCACCCTTCGCCATGCGCTCGCGCAT
CGCCGCGCGCCTGAAGGTGATGGCGCGGCTGGACATCTCCGAACGCCGCCTGCCGCAGGATGGTGCCATCAAGCTGAAGG
TATCCAAGACCCGCTCGATCGATTTCCGCGTGAACTCACTGCCGACCGTGTACGGCGAGAAGATCGTGCTGCGTATTCTC
GATCCTACCTCGGCGCAGATCGGCATCGATGCACTGGGTTTCACGCCGGACCAGCGCACGCTATACGAAGGCGTGCTGGA
TCATCCTCAGGGCATGATCCTGGTGACCGGCCCCACCGGTAGCGGCAAGACGGTGACGCTCTATACCGGCCTGAACATTC
TCAACGAGGTACAGCGCAACATCTGTACCGCCGAGGATCCGGTGGAGATCAAGGTGCCCGGGGTCAATCAGGTCAACGTG
CTGCCCAAGATCGGCCTGGACTTCGCCAGCGCGCTGCGCGCTTTCCTGCGCCAGGATCCGGACGTGGTGATGGTGGGCGA
GATACGCGACTTGGAGACTGCCGAGATCGCAGTGAAGGCTTCTCAGACCGGTCATTTGGTGCTCTCCACGGTACACACCA
ACTCCGCCGCGGAGACACTGACCCGCCTGGCCAACATGGGCGTGCCGGCGTTCAACATTGCCAGCTCGGTGAGCCTGATC
ATCGCCCAACGCCTGGCACGGCGGCTGTGCAAGCACTGTCGCCAGGAGGCGGAGCTACCCCGCGAGGTGCTGCTACAGGA
GGGCTTCACCGAACAGGAGGTGGACAGCGCCACGGTATATGAAGCCGTCGGCTGCAAGCACTGCACGCATGGCTACAAGG
GGCGGGTGGGCATCTACGAAGTGGTGCCGATCAGCAATGCCATGAGCCAACTGATCATGCGCAACGGCAACTCGATGGAC
TTCGACAGCCTGGCCCGCCAGGAAGGCCACCCGGACCTGCGTCGCAGCGGGCTGTTGAAGGTAATGCAGGGGATCACTAG
CCTGACCGAGATCAATCGCGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(221-488)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.371

95.812

0.521
  pilB Acinetobacter baylyi ADP1

51.712

97.822

0.506
  pilB Acinetobacter baumannii D1279779

52.632

95.477

0.503
  pilB Vibrio campbellii strain DS40M4

47.478

96.315

0.457
  pilB Vibrio cholerae strain A1552

50.093

90.285

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

47.585

93.635

0.446
  pilF Neisseria gonorrhoeae MS11

46.503

95.812

0.446