Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HNO52_RS11705 Genome accession   NZ_CP053382
Coordinates   2515329..2517128 (+) Length   599 a.a.
NCBI ID   WP_197565485.1    Uniprot ID   A0A7M1QC44
Organism   Halomonas sp. MCCC 1A13316     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2510329..2522128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNO52_RS11690 (HNO52_11690) - 2511016..2512806 (-) 1791 WP_197565483.1 PilX N-terminal domain-containing pilus assembly protein -
  HNO52_RS11695 (HNO52_11695) - 2512925..2514346 (-) 1422 WP_197565484.1 O-antigen ligase family protein -
  HNO52_RS21080 - 2514754..2514885 (-) 132 Protein_2312 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HNO52_RS11705 (HNO52_11705) pilB 2515329..2517128 (+) 1800 WP_197565485.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNO52_RS11710 (HNO52_11710) pilC 2517244..2518476 (+) 1233 WP_197565486.1 type II secretion system F family protein Machinery gene
  HNO52_RS11715 (HNO52_11715) pilD 2518486..2519337 (+) 852 WP_197565487.1 A24 family peptidase Machinery gene
  HNO52_RS11720 (HNO52_11720) coaE 2519402..2520007 (+) 606 WP_197565488.1 dephospho-CoA kinase -
  HNO52_RS11725 (HNO52_11725) yacG 2520067..2520294 (+) 228 WP_197565489.1 DNA gyrase inhibitor YacG -
  HNO52_RS11730 (HNO52_11730) - 2520351..2521160 (+) 810 WP_197569207.1 DNA replication terminus site-binding protein -

Sequence

Protein


Download         Length: 599 a.a.        Molecular weight: 65700.26 Da        Isoelectric Point: 5.4421

>NTDB_id=444634 HNO52_RS11705 WP_197565485.1 2515329..2517128(+) (pilB) [Halomonas sp. MCCC 1A13316]
MNDYRTAPDKTRAHARDGESAPTQPVANLDGLRGFARRLVEYGLLSRAAAERAEYEAREAETSLLQHVVDNGLVTARQGT
LCAAWEYGLPLVDLDALRLASLPPANEYPEKLLRKLCVVPLSRRSHRLTVAVPYPSTLALLDELQFATGLGIEAVLCPVD
QLMPVLEAYLAQHDSSMMDELAGVDDAVSELEFGEGVDLSDERADDAALSGADDAPIVKFVNKVLLDAIRRGASDIHFEP
YETSYRVRMRVDGMLMESARPPFAMRSRIAARLKVMARLDISERRLPQDGAIKLKVSKTRSIDFRVNSLPTVYGEKIVLR
ILDPASAQIGIDALGFTPEQRALYEGVLDQPQGMILVTGPTGSGKTVTLYTGLNILNEVQRNICTAEDPVEIKVPGVNQV
NVLPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVPAFNIASSVS
LIIAQRLARRLCKHCRQEAELPREVLLQEGFTEQEVDNATVYEAVGCKHCTHGYKGRVGIYEVVPISTAMSQLIMRNGNS
MDFDTLARQEGHPDLRRSGLLKVMQGITSLTEINRITKD

Nucleotide


Download         Length: 1800 bp        

>NTDB_id=444634 HNO52_RS11705 WP_197565485.1 2515329..2517128(+) (pilB) [Halomonas sp. MCCC 1A13316]
ATGAACGACTACCGAACAGCACCCGACAAGACCCGTGCCCACGCCCGAGATGGCGAGAGCGCCCCCACGCAGCCCGTGGC
CAACCTCGACGGCCTGCGCGGTTTCGCGCGCAGGCTGGTGGAGTACGGGCTGCTTTCACGCGCGGCGGCCGAGCGGGCGG
AGTACGAGGCGCGCGAGGCGGAGACCAGCCTGCTGCAGCATGTGGTCGATAACGGCCTGGTCACGGCTCGCCAGGGTACG
CTGTGCGCTGCCTGGGAATACGGCCTGCCCTTGGTCGATCTGGACGCGCTGCGCCTGGCGAGCCTACCCCCGGCAAACGA
ATATCCCGAGAAGCTGCTGCGCAAGCTGTGCGTGGTACCGCTCAGCCGCCGCAGCCACCGGCTAACCGTTGCCGTGCCTT
ACCCTTCCACCCTGGCCCTGCTCGACGAGCTGCAATTCGCCACCGGCCTGGGCATCGAAGCCGTGCTGTGCCCGGTGGAT
CAGCTGATGCCGGTACTTGAGGCCTACCTGGCCCAGCACGATTCCAGCATGATGGACGAGCTGGCAGGGGTCGATGACGC
GGTAAGCGAGCTGGAATTTGGCGAAGGGGTCGACCTCAGCGACGAGCGCGCCGACGATGCCGCCCTGAGCGGTGCCGACG
ATGCGCCGATCGTCAAGTTCGTCAACAAGGTGTTGCTGGATGCCATTCGCCGCGGCGCCTCTGACATACACTTCGAGCCC
TACGAGACCAGTTATCGCGTGCGCATGCGCGTGGACGGCATGCTGATGGAGTCGGCACGCCCGCCCTTCGCCATGCGTTC
GCGCATCGCCGCCCGCCTCAAGGTAATGGCGCGGCTGGATATTTCCGAACGCCGCCTACCGCAGGATGGCGCGATCAAGC
TGAAGGTATCGAAGACCCGCTCGATCGATTTCCGCGTGAACTCGCTGCCAACCGTATACGGCGAGAAGATCGTACTGCGT
ATTCTCGACCCTGCCTCGGCGCAGATCGGCATCGATGCGCTGGGCTTCACACCGGAACAGCGCGCCCTGTACGAAGGCGT
GCTTGACCAACCCCAGGGCATGATACTGGTTACCGGCCCTACCGGTAGCGGCAAAACGGTAACGCTCTATACCGGGCTGA
ACATCCTCAACGAGGTGCAGCGCAACATCTGTACCGCCGAAGACCCGGTGGAGATCAAGGTCCCGGGGGTCAACCAGGTC
AACGTGCTGCCCAAGATCGGCCTGGATTTCGCCAGCGCCCTCCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGT
GGGTGAGATCCGCGACCTGGAGACGGCCGAGATCGCGGTGAAGGCGTCACAGACTGGCCACCTGGTGCTCTCCACGGTAC
ACACCAATTCAGCCGCAGAGACCCTCACCCGCCTGGCTAACATGGGTGTGCCGGCTTTCAACATTGCCAGCTCCGTTAGC
CTGATCATCGCTCAGCGCCTGGCCCGCCGGCTGTGCAAGCACTGCCGCCAGGAGGCGGAGCTTCCCCGCGAGGTGTTGCT
GCAGGAAGGCTTCACCGAGCAGGAGGTGGATAACGCCACGGTGTATGAAGCAGTCGGCTGCAAGCACTGCACGCATGGCT
ACAAGGGCCGGGTGGGGATCTATGAAGTGGTGCCGATCAGCACGGCCATGAGCCAGCTGATCATGCGCAACGGCAACTCG
ATGGACTTCGACACCCTGGCTCGCCAGGAGGGGCATCCGGACCTGCGCCGCAGCGGGCTATTGAAGGTGATGCAGGGGAT
TACCAGTTTGACTGAAATCAACAGGATCACAAAAGACTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-172)

Type II/IV secretion system protein (T2SSE)

(223-490)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.386

95.159

0.518
  pilB Acinetobacter baylyi ADP1

52.982

95.159

0.504
  pilB Acinetobacter baumannii D1279779

51.761

94.825

0.491
  pilB Vibrio cholerae strain A1552

50.652

89.649

0.454
  pilB Vibrio campbellii strain DS40M4

46.914

94.658

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

47.585

93.322

0.444
  pilF Neisseria gonorrhoeae MS11

46.14

95.159

0.439