NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	HNO52_RS11715	Genome accession	NZ_CP053382
Coordinates	2518486..2519337 (+)	Length	283 a.a.
NCBI ID	WP_197565487.1	Uniprot ID	A0A7M1QCZ3
Organism	Halomonas sp. MCCC 1A13316
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2513486..2524337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HNO52_RS21080	-	2514754..2514885 (-)	132	Protein_2312	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
HNO52_RS11705 (HNO52_11705)	pilB	2515329..2517128 (+)	1800	WP_197565485.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HNO52_RS11710 (HNO52_11710)	pilC	2517244..2518476 (+)	1233	WP_197565486.1	type II secretion system F family protein	Machinery gene
HNO52_RS11715 (HNO52_11715)	pilD	2518486..2519337 (+)	852	WP_197565487.1	A24 family peptidase	Machinery gene
HNO52_RS11720 (HNO52_11720)	coaE	2519402..2520007 (+)	606	WP_197565488.1	dephospho-CoA kinase	-
HNO52_RS11725 (HNO52_11725)	yacG	2520067..2520294 (+)	228	WP_197565489.1	DNA gyrase inhibitor YacG	-
HNO52_RS11730 (HNO52_11730)	-	2520351..2521160 (+)	810	WP_197569207.1	DNA replication terminus site-binding protein	-
HNO52_RS11735 (HNO52_11735)	-	2521186..2522556 (-)	1371	WP_197565490.1	MFS transporter	-
HNO52_RS11740 (HNO52_11740)	-	2522563..2523321 (-)	759	WP_197565491.1	tellurite resistance TerB family protein	-
HNO52_RS11745 (HNO52_11745)	-	2523398..2523997 (-)	600	WP_197565492.1	2-hydroxychromene-2-carboxylate isomerase	-

Sequence

Protein

Download Length: 283 a.a. Molecular weight: 30774.05 Da Isoelectric Point: 5.7603

                                >NTDB_id=444636 HNO52_RS11715 WP_197565487.1 2518486..2519337(+) (pilD) [Halomonas sp. MCCC 1A13316]

MLVDVPPAILWPLVAVLGLCLGSFLNVVIVRLPVMLMLGWRAEAREALELPEEESHRFNLLVPRSICPQCETPITWHDNL

PVVGWFKRRGRCAACEARISPQYPLVELAGAGLALAVLALYGATWQGLFVFGACLALLALAVIDLRTQLLPDMMTLPLLW

AGLLYQLLFQPLMLTDAVIGAMAGYLALWSVYWLFKLVTGKEGMGYGDFKLLAALGAWLGWQYLPMVLILSAGVGAVVGI

AIQLALPRLRGAPMPFGPYLASAGWVALLVGEPLMAAYVDMAF

Nucleotide

Download Length: 852 bp

                                >NTDB_id=444636 HNO52_RS11715 WP_197565487.1 2518486..2519337(+) (pilD) [Halomonas sp. MCCC 1A13316]

TTGCTGGTTGACGTTCCCCCCGCCATTCTTTGGCCACTTGTGGCCGTGCTGGGTCTTTGCCTGGGCAGTTTCCTCAACGT

GGTTATCGTACGTCTGCCGGTCATGCTGATGCTGGGCTGGCGCGCCGAGGCCCGCGAGGCGCTTGAGCTGCCCGAAGAGG

AGTCGCACCGCTTCAATCTGCTGGTGCCGCGCTCGATATGCCCCCAGTGCGAGACGCCTATTACCTGGCATGACAACTTG

CCGGTCGTCGGCTGGTTCAAGCGACGCGGCCGCTGTGCTGCCTGTGAAGCGCGCATCAGCCCGCAGTATCCTCTAGTGGA

GCTGGCCGGTGCCGGCCTGGCACTGGCTGTACTGGCGCTTTACGGGGCCACCTGGCAAGGCCTGTTCGTCTTCGGCGCCT

GCCTGGCGCTGCTGGCCCTGGCCGTGATCGACCTGCGTACCCAGTTGCTGCCGGACATGATGACCCTGCCGCTGCTGTGG

GCCGGGCTGCTTTACCAGTTGCTATTCCAGCCGCTGATGTTGACTGACGCCGTGATCGGCGCCATGGCGGGTTATCTCGC

CCTGTGGAGCGTCTACTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTACGGTGACTTCAAGCTGCTGGCGG

CCCTGGGCGCCTGGCTGGGCTGGCAGTACCTGCCGATGGTGTTGATCCTTTCCGCCGGCGTGGGTGCCGTCGTGGGCATC

GCCATTCAGCTCGCCCTGCCCCGCCTTCGCGGCGCACCGATGCCCTTCGGACCCTACCTGGCCAGCGCCGGCTGGGTGGC

GCTGCTGGTAGGCGAGCCGCTGATGGCCGCCTATGTCGACATGGCGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(134-240)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(16-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	52.837	99.647	0.527
pilD	Vibrio campbellii strain DS40M4	50.735	96.113	0.488
pilD	Acinetobacter baumannii D1279779	47.232	95.76	0.452
pilD	Acinetobacter nosocomialis M2	46.494	95.76	0.445
pilD	Neisseria gonorrhoeae MS11	45.896	94.7	0.435

Detailed information NTG0444636