Detailed information NTG0444558

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HNO13_RS12365
• Genome accession: NZ_CP053377
• Coordinates: 2589836..2590450 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain EN01
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2584836..2595450

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HNO13_RS12335 (HNO13_12370)	rpsT	2585136..2585402 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HNO13_RS12340 (HNO13_12375)	holA	2585419..2586460 (-)	1042	Protein_2404	DNA polymerase III subunit delta	-
HNO13_RS12345 (HNO13_12380)	-	2586500..2586654 (-)	155	Protein_2405	hypothetical protein	-
HNO13_RS12350 (HNO13_12385)	-	2586695..2586829 (+)	135	WP_003152870.1	YqzM family protein	-
HNO13_RS12355 (HNO13_12390)	comEC	2586848..2589199 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HNO13_RS12360 (HNO13_12395)	-	2589200..2589769 (-)	570	WP_003152868.1	ComE operon protein 2	-
HNO13_RS12365 (HNO13_12400)	comEA	2589836..2590450 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
HNO13_RS12370 (HNO13_12405)	comER	2590509..2591330 (+)	822	WP_014305449.1	late competence protein ComER	-
HNO13_RS12375 (HNO13_12410)	-	2591399..2592136 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
HNO13_RS12380 (HNO13_12415)	rsfS	2592133..2592489 (-)	357	WP_003152864.1	ribosome silencing factor	-
HNO13_RS12385 (HNO13_12420)	yqeK	2592507..2593067 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HNO13_RS12390 (HNO13_12425)	-	2593057..2593626 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
HNO13_RS12395 (HNO13_12430)	yhbY	2593637..2593927 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HNO13_RS12400 (HNO13_12435)	aroE	2593921..2594763 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=444558 HNO13_RS12365 WP_041482377.1 2589836..2590450(-) (comEA) [Bacillus velezensis strain EN01]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=444558 HNO13_RS12365 WP_041482377.1 2589836..2590450(-) (comEA) [Bacillus velezensis strain EN01]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363