Detailed information NTG0444482

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HNO12_RS12360
• Genome accession: NZ_CP053376
• Coordinates: 2587418..2588032 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain WF02
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582418..2593032

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HNO12_RS12330 (HNO12_12365)	rpsT	2582718..2582984 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HNO12_RS12335 (HNO12_12370)	holA	2583001..2584042 (-)	1042	Protein_2403	DNA polymerase III subunit delta	-
HNO12_RS12340 (HNO12_12375)	-	2584082..2584236 (-)	155	Protein_2404	hypothetical protein	-
HNO12_RS12345 (HNO12_12380)	-	2584277..2584411 (+)	135	WP_003152870.1	YqzM family protein	-
HNO12_RS12350 (HNO12_12385)	comEC	2584430..2586781 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HNO12_RS12355 (HNO12_12390)	-	2586782..2587351 (-)	570	WP_003152868.1	ComE operon protein 2	-
HNO12_RS12360 (HNO12_12395)	comEA	2587418..2588032 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
HNO12_RS12365 (HNO12_12400)	comER	2588091..2588912 (+)	822	WP_014305449.1	late competence protein ComER	-
HNO12_RS12370 (HNO12_12405)	-	2588981..2589718 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
HNO12_RS12375 (HNO12_12410)	rsfS	2589715..2590071 (-)	357	WP_003152864.1	ribosome silencing factor	-
HNO12_RS12380 (HNO12_12415)	yqeK	2590089..2590649 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HNO12_RS12385 (HNO12_12420)	-	2590639..2591208 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
HNO12_RS12390 (HNO12_12425)	yhbY	2591219..2591509 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HNO12_RS12395 (HNO12_12430)	aroE	2591503..2592345 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=444482 HNO12_RS12360 WP_041482377.1 2587418..2588032(-) (comEA) [Bacillus amyloliquefaciens strain WF02]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=444482 HNO12_RS12360 WP_041482377.1 2587418..2588032(-) (comEA) [Bacillus amyloliquefaciens strain WF02]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363