Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HKW95_RS02195 Genome accession   NZ_CP053058
Coordinates   486130..488202 (+) Length   690 a.a.
NCBI ID   WP_023970130.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain FS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481130..493202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW95_RS02175 (HKW95_02170) pilM 483360..484412 (+) 1053 WP_023970126.1 pilus assembly protein PilM Machinery gene
  HKW95_RS02180 (HKW95_02175) - 484412..484978 (+) 567 WP_023970127.1 PilN domain-containing protein -
  HKW95_RS02185 (HKW95_02180) - 484975..485592 (+) 618 WP_023970128.1 type 4a pilus biogenesis protein PilO -
  HKW95_RS02190 (HKW95_02185) - 485589..486116 (+) 528 WP_023970129.1 pilus assembly protein PilP -
  HKW95_RS02195 (HKW95_02190) pilQ 486130..488202 (+) 2073 WP_023970130.1 type IV pilus secretin PilQ Machinery gene
  HKW95_RS02200 (HKW95_02195) aroK 488207..488725 (+) 519 WP_009041824.1 shikimate kinase AroK -
  HKW95_RS02205 (HKW95_02200) aroB 488874..489974 (+) 1101 WP_023970131.1 3-dehydroquinate synthase -
  HKW95_RS02210 (HKW95_02205) - 489985..491565 (+) 1581 WP_023970132.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74715.79 Da        Isoelectric Point: 5.3037

>NTDB_id=442558 HKW95_RS02195 WP_023970130.1 486130..488202(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain FS2]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGPRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQLAVASGRAKATASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFARARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDQRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKTDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=442558 HKW95_RS02195 WP_023970130.1 486130..488202(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain FS2]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCTCTGGATGTCGCGGCGCTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAACTGGCGAACAAGAGCCGCGAC
CTGGGCAATGGCAATGCACGCAGCGTGACAGTGGTCGAATCGGGGCCGCGTACCCGGGTGGTGGTCAACCTGGTCGCACT
GGCGCCCTACAGCACTCGAGTCGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCAC
CGGGGCAGCTGGCTGTCGCGTCCGGGCGGGCCAAGGCCACGGCTTCGGCGGGACGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGTGAAGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTTACCTTCGCCAGGGCCCGTCTGCCAGAACCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTTGATCTGGTGCTCAAGACCAAGGGGCTGGACCAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGACCTCGAGCCGCTGCGGCGCGAGCTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGACCGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAACTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGTGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTACCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATAGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(298-344)

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.21

100

0.728
  comQ Acinetobacter baylyi ADP1

39.698

100

0.419
  pilQ Acinetobacter baumannii D1279779

37.718

100

0.407
  pilQ Acinetobacter baumannii strain A118

37.718

100

0.407
  pilQ Legionella pneumophila strain Lp02

37.89

97.536

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.957

0.368