Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HH212_RS03360 Genome accession   NZ_CP051685
Coordinates   789766..791493 (+) Length   575 a.a.
NCBI ID   WP_169434088.1    Uniprot ID   A0A7Z2VTX1
Organism   Massilia forsythiae strain GN2-R2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 784766..796493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH212_RS03340 (HH212_03340) - 785407..785805 (+) 399 WP_169434084.1 hypothetical protein -
  HH212_RS03345 (HH212_03345) - 785878..787461 (+) 1584 WP_169434085.1 methyl-accepting chemotaxis protein -
  HH212_RS03350 (HH212_03350) - 787806..789047 (+) 1242 WP_169434086.1 hypothetical protein -
  HH212_RS03355 (HH212_03355) - 789059..789466 (+) 408 WP_169434087.1 hypothetical protein -
  HH212_RS03360 (HH212_03360) pilB 789766..791493 (+) 1728 WP_169434088.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HH212_RS03365 (HH212_03365) pilC 791504..792739 (+) 1236 WP_169434089.1 type II secretion system F family protein Machinery gene
  HH212_RS03370 (HH212_03370) - 792795..793175 (-) 381 WP_169434090.1 translational machinery protein -
  HH212_RS03375 (HH212_03375) - 793322..794008 (-) 687 WP_169434091.1 dihydrofolate reductase family protein -
  HH212_RS03380 (HH212_03380) - 794151..794417 (-) 267 WP_169434092.1 PAAR domain-containing protein -
  HH212_RS03385 (HH212_03385) - 794443..796278 (-) 1836 WP_169434093.1 DUF2875 family protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 61851.49 Da        Isoelectric Point: 5.1795

>NTDB_id=440449 HH212_RS03360 WP_169434088.1 789766..791493(+) (pilB) [Massilia forsythiae strain GN2-R2]
MAAVLPTTTTGSMLPGLARALVQSGKLGMPQADTLQKKAANDKVGFIDALLGSGAIDARNLALFCAETFGYPLLDLSAFS
PEMLPANAIDAKLMQTLRVVPLSKRGNKMSVALSDPTNSQALDQIKFQSEASVEPVIVAHDVLQALLASLAKSGDQSLND
LVGEEGDIEFAEEESQAAAAEAPVSEVEDAPIVRFLNKVLMDAITLGASDLHFEPYEKQYRIRLRVDGVLRDHMAPPLAI
RDKLVSRIKVLAKLDISEKRVPQDGRMRLVLSPTRTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKALLV
DAITRPYGMVLVTGPTGSGKTVSLYSCLNLLNKPGINISTAEDPAEINLPGVNQVNVNEKAGLTFPIALKAFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPLEVGRDVL
KSAGFRDADLEGDWKPYGPIGCERCLGSGYKGRVGIYQIMPITEAIEALILGHGNAMQIAAQAELEGVNSLRRSGLMKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=440449 HH212_RS03360 WP_169434088.1 789766..791493(+) (pilB) [Massilia forsythiae strain GN2-R2]
ATGGCCGCAGTCCTTCCCACCACCACCACTGGCAGCATGCTGCCCGGGTTGGCGCGCGCCCTGGTCCAGTCCGGCAAGCT
CGGCATGCCGCAGGCGGACACCCTGCAAAAGAAGGCGGCCAACGACAAGGTCGGCTTCATCGACGCCCTGCTCGGCTCGG
GCGCGATCGATGCGCGCAACCTGGCGCTGTTCTGCGCCGAGACCTTCGGCTACCCGCTGCTCGACCTGTCCGCCTTCAGC
CCGGAGATGCTGCCCGCCAACGCCATCGACGCCAAGCTGATGCAGACGCTGCGCGTGGTGCCGCTGTCCAAGCGCGGCAA
CAAGATGTCGGTGGCGCTGTCCGACCCGACCAATTCCCAGGCCCTCGACCAGATCAAGTTCCAGAGCGAAGCCTCGGTCG
AGCCGGTGATCGTCGCCCACGACGTGCTGCAGGCGCTGCTGGCCAGCCTGGCCAAGAGCGGCGACCAGAGCCTGAACGAC
CTGGTGGGCGAGGAAGGCGACATCGAATTCGCCGAAGAGGAATCGCAGGCCGCGGCAGCGGAAGCGCCGGTGTCCGAAGT
CGAGGACGCGCCCATCGTGCGCTTCCTGAACAAGGTGCTGATGGACGCGATCACGCTGGGCGCATCCGACCTGCACTTCG
AACCCTACGAAAAGCAGTACCGCATCCGCCTGCGCGTGGACGGCGTGCTGCGCGACCACATGGCGCCGCCGCTGGCGATC
CGCGACAAGCTGGTCTCGCGCATCAAGGTGCTGGCCAAACTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCAT
GCGCCTGGTGCTGTCGCCGACCCGCACCATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAAAAGACCGTCA
TGCGTATCCTCGACGCCACACAGGCACAGATGGGCATCGACGCGCTCGGCTACGACCCCGACCAGAAGGCGCTGCTGGTG
GACGCCATCACCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACGGTGTCCCTGTACAGCTG
CCTGAATTTGCTGAACAAACCCGGCATCAACATCTCGACCGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACC
AGGTCAACGTCAACGAAAAGGCGGGACTGACCTTTCCGATCGCCCTGAAGGCGTTCCTGCGCCAGGACCCGGACATCATC
ATGGTGGGCGAGATCCGCGACCTGGAAACCGCCGACATCGCGATCAAGGCGGCCCAGACCGGCCACATGGTGTTCTCGAC
GCTGCACACCAACGACGCCCCGTCCACCCTGACGCGCCTCATGAACATGGGCGTGGCGCCATTCAACATCGCCTCCTCCG
TGATCTTGATCACGGCGCAGCGCCTGGCGCGCCGCCTGTGCACCTGCAAGCAGCCGCTCGAGGTCGGGCGCGACGTATTG
AAATCGGCGGGTTTCCGCGACGCCGACCTGGAAGGCGACTGGAAGCCGTACGGCCCGATCGGCTGCGAGCGCTGCCTGGG
TTCCGGCTACAAGGGCCGCGTCGGCATCTACCAGATCATGCCGATCACGGAAGCGATCGAAGCCTTGATCCTGGGGCACG
GCAACGCCATGCAGATCGCCGCCCAGGCCGAGCTGGAAGGCGTGAATTCTCTGCGTCGCTCCGGCCTGATGAAGGTGAAA
CAAGGACTCACCAGCCTCGAGGAAGTCCTCGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-153)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2VTX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.954

98.957

0.534
  pilB Acinetobacter baumannii D1279779

53.415

99.304

0.53
  pilF Neisseria gonorrhoeae MS11

53.065

99.304

0.527
  pilB Legionella pneumophila strain ERS1305867

49.023

97.913

0.48
  pilB Vibrio campbellii strain DS40M4

45.629

99.478

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

45.15

98.609

0.445
  pilB Vibrio cholerae strain A1552

44.76

97.913

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.072

96.348

0.386
  pilF Thermus thermophilus HB27

37.857

97.391

0.369