Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HH213_RS16505 Genome accession   NZ_CP051684
Coordinates   3613194..3614924 (-) Length   576 a.a.
NCBI ID   WP_169112925.1    Uniprot ID   -
Organism   Duganella dendranthematis strain AF9R3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3608194..3619924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH213_RS16475 (HH213_16475) - 3608521..3608694 (-) 174 WP_110847246.1 DNA gyrase inhibitor YacG -
  HH213_RS16480 (HH213_16480) zapD 3608706..3609461 (-) 756 WP_161056074.1 cell division protein ZapD -
  HH213_RS16485 (HH213_16485) coaE 3609563..3610171 (-) 609 WP_169112921.1 dephospho-CoA kinase -
  HH213_RS16490 (HH213_16490) pilD 3610168..3611040 (-) 873 WP_169112922.1 A24 family peptidase Machinery gene
  HH213_RS16495 (HH213_16495) - 3611196..3611975 (+) 780 WP_169112923.1 sensor histidine kinase -
  HH213_RS16500 (HH213_16500) pilC 3611958..3613181 (-) 1224 WP_169112924.1 type II secretion system F family protein Machinery gene
  HH213_RS16505 (HH213_16505) pilF 3613194..3614924 (-) 1731 WP_169112925.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HH213_RS16510 (HH213_16510) - 3615056..3616336 (-) 1281 WP_110847239.1 HlyC/CorC family transporter -
  HH213_RS16515 (HH213_16515) - 3616466..3616708 (-) 243 WP_229263029.1 FlxA-like family protein -
  HH213_RS16520 (HH213_16520) - 3616831..3617061 (-) 231 WP_169112927.1 pentapeptide MXKDX repeat protein -
  HH213_RS16525 (HH213_16525) - 3617116..3617847 (-) 732 WP_371875715.1 molybdopterin-dependent oxidoreductase -
  HH213_RS16530 (HH213_16530) - 3617921..3618523 (-) 603 WP_110847235.1 cytochrome b/b6 domain-containing protein -
  HH213_RS16535 (HH213_16535) - 3618638..3619222 (+) 585 WP_169112928.1 sigma-70 family RNA polymerase sigma factor -
  HH213_RS16540 (HH213_16540) - 3619219..3619860 (+) 642 WP_169112929.1 NrsF family protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62133.81 Da        Isoelectric Point: 5.2944

>NTDB_id=440444 HH213_RS16505 WP_169112925.1 3613194..3614924(-) (pilF) [Duganella dendranthematis strain AF9R3]
MAAVLPNAVPSAPMPGLARALMQAGRLSAVQAEALQKKSVADKQAFIDVLLSSGAIESKALAAFCAETFAYPLLDVNTLS
VDALPAKIIDNKLMQTQRVIALSKRGNKMSVAISDPTNTQALDQIKFQTESTVEPVIVAHDALLQLLARLNKSSEQSIAD
LAGDEAEIQFAEEEEVASAAADNAANEVEDAPVVRFLNKMLMDAVNLGASDLHFEPFEKFYRIRFRVDGVLMEHAQPPIS
IKEKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTVDLRISTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQRELL
LDAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVASFNIASSVILITAQRLARRLCSCKQPVDITPEL
LIKAGFKPEELDGSWKPYGPVGCERCNGGGYKGRVGIYQIMPITPAIENLILAHGNAMQIAAQSEAEGVKSLRQSGLVKV
KAGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=440444 HH213_RS16505 WP_169112925.1 3613194..3614924(-) (pilF) [Duganella dendranthematis strain AF9R3]
ATGGCCGCAGTCCTCCCGAACGCAGTGCCAAGCGCCCCGATGCCGGGACTGGCGCGGGCATTGATGCAAGCAGGACGGCT
TTCCGCCGTCCAGGCCGAAGCCTTGCAAAAAAAATCAGTCGCGGACAAGCAGGCTTTCATCGACGTGCTGCTGTCCAGCG
GCGCGATTGAATCGAAGGCGCTGGCCGCCTTCTGCGCTGAAACCTTCGCCTACCCGCTGCTGGACGTCAATACGCTGAGC
GTCGACGCCCTGCCCGCCAAGATCATCGACAACAAGCTGATGCAGACGCAGCGCGTGATCGCGCTGTCCAAGCGCGGCAA
CAAGATGTCAGTGGCGATTTCCGATCCGACCAACACGCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGTCCACCGTCG
AACCGGTGATCGTGGCGCACGACGCGCTGCTGCAACTACTGGCGCGCCTCAACAAGAGCAGCGAACAAAGCATTGCCGAC
CTGGCCGGCGACGAAGCCGAAATCCAGTTCGCCGAGGAAGAGGAAGTTGCCAGCGCCGCCGCCGACAACGCCGCCAACGA
AGTCGAGGACGCGCCGGTGGTGCGCTTCCTCAACAAGATGCTGATGGACGCCGTCAACCTCGGCGCTTCCGACTTGCACT
TCGAGCCGTTTGAAAAGTTCTACCGCATCCGCTTCCGCGTCGACGGCGTGCTGATGGAGCATGCGCAGCCGCCGATTTCG
ATCAAGGAAAAACTGGTCTCGCGCATCAAGGTGCTGGCCAAGCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCG
CATGCGCCTGATCGTCTCGCCGACCAAGACCGTCGACCTGCGCATCTCGACGCTGCCGACGCTGTTCGGCGAAAAGACCG
TGATGCGGATTCTCGACGCCACCCAAGCGCAGATGGGCATCGACTCGCTCGGCTACGATCCCGACCAGCGCGAACTGCTG
CTGGACGCCATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTGTACAC
CTGCCTCAACATTCTGAACAAGCCTGGCATCAACATCTCCACCGCCGAAGATCCGGCCGAGATCAACCTGCCCGGCGTGA
ACCAGGTCAATGTCAACGACAAGGCCGGCCTGACTTTCCCGGTGGCGCTGAAGTCCTTCCTGCGCCAGGACCCGGACATC
ATCATGGTCGGCGAGATCCGCGACCTGGAGACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGTTCTC
CACGCTGCACACCAACGACGCGCCGTCGACGCTGACTCGGCTGATGAACATGGGCGTGGCGTCGTTCAATATCGCCTCGT
CGGTGATCCTGATTACCGCCCAGCGCCTGGCGCGCCGCCTGTGCAGCTGCAAGCAGCCGGTCGACATCACGCCCGAACTG
CTGATCAAGGCCGGTTTCAAGCCGGAGGAGCTGGACGGCAGCTGGAAACCCTACGGACCGGTCGGTTGTGAACGCTGCAA
CGGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGATCATGCCGATCACGCCCGCGATCGAAAACTTGATCCTGGCGC
ACGGCAACGCGATGCAGATCGCCGCGCAGTCGGAGGCGGAAGGCGTCAAGTCGCTGCGCCAGTCCGGGCTGGTCAAGGTC
AAGGCCGGCCTCACCAGCCTGGAAGAAGTGCTCGGCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.078

97.917

0.53
  pilB Acinetobacter baumannii D1279779

51.568

99.653

0.514
  pilB Acinetobacter baylyi ADP1

52.22

97.743

0.51
  pilB Legionella pneumophila strain ERS1305867

47.594

97.396

0.464
  pilB Vibrio cholerae strain A1552

45.31

98.09

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.293

97.743

0.443
  pilB Vibrio campbellii strain DS40M4

44.17

98.264

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.817

94.618

0.377
  pilF Thermus thermophilus HB27

39.455

95.486

0.377