Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HH196_RS05750 Genome accession   NZ_CP051666
Coordinates   1215626..1217353 (+) Length   575 a.a.
NCBI ID   WP_169451203.1    Uniprot ID   -
Organism   Marinobacterium sp. LSUCC0821     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1210626..1222353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HH196_RS05720 (HH196_05720) - 1211502..1212218 (+) 717 WP_169451198.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HH196_RS05725 (HH196_05725) ampD 1212189..1212794 (-) 606 WP_169451199.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HH196_RS05730 (HH196_05730) nadC 1212861..1213700 (+) 840 WP_169451200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HH196_RS05735 (HH196_05735) - 1214190..1214366 (+) 177 WP_169451201.1 hypothetical protein -
  HH196_RS05740 (HH196_05740) - 1214556..1214909 (-) 354 WP_169451202.1 hypothetical protein -
  HH196_RS11525 - 1215240..1215351 (-) 112 Protein_1113 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HH196_RS05750 (HH196_05750) pilB 1215626..1217353 (+) 1728 WP_169451203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HH196_RS05755 (HH196_05755) pilC 1217365..1218600 (+) 1236 WP_248276828.1 type II secretion system F family protein Machinery gene
  HH196_RS05760 (HH196_05760) yacG 1218665..1218871 (-) 207 WP_169451204.1 DNA gyrase inhibitor YacG -
  HH196_RS05765 (HH196_05765) coaE 1218873..1219469 (-) 597 WP_169451205.1 dephospho-CoA kinase -
  HH196_RS05770 (HH196_05770) mutT 1219462..1219869 (-) 408 WP_169451206.1 8-oxo-dGTP diphosphatase MutT -
  HH196_RS05775 (HH196_05775) argJ 1219895..1221109 (-) 1215 WP_169451207.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63328.78 Da        Isoelectric Point: 5.5138

>NTDB_id=440358 HH196_RS05750 WP_169451203.1 1215626..1217353(+) (pilB) [Marinobacterium sp. LSUCC0821]
MGLQASQPLLNGLAKRLVNTGVIDSEATKRALSESRAAQKSFISYLIEKRLISARRAAVAASDEFGLPILDLDSFMLDAI
PKELIDAKLIAKHQVIPLIKRENRLYVGISDPSNIRALDELRFSTGIPTEAVIVEEDKLTRKIELLIDSQDDGTAALGDL
ADSDLDNLELGDTERRTEEDSVADATSDAPIVRFVNKVLLDAIKNGASDIHFEPYEKSYRVRCRIDGILQEVSNPPINLA
ARLSARLKVMSNMDIAERRVPQDGRIKLKVSAKRSIDFRVNTLPTLWGEKIVLRILDPTSATIGVDALGFEPEQRELFMS
VLRRPQGMVLVTGPTGSGKTVTLYTGLNILNEEERNISTAEDPVEINMEGVNQVHVNPKVGLTFAEALRAFLRQDPDVVM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVTLIIAQRLARRLCSSCKTQVDLPRATL
LEEGFTEEQLEKLTIFEHNPEGCPRCNRGYRGRVGIYEMLKVSPEIAECIMNNGNAIEILKVAKSEGFATLRSAGLRKVA
AGVTSLEEMNRVIKT

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=440358 HH196_RS05750 WP_169451203.1 1215626..1217353(+) (pilB) [Marinobacterium sp. LSUCC0821]
ATGGGCTTGCAAGCATCTCAGCCACTACTGAATGGACTCGCAAAAAGACTGGTGAATACCGGTGTTATTGATAGTGAAGC
AACTAAACGTGCACTCAGTGAATCCCGTGCCGCGCAGAAGTCCTTTATATCCTATCTGATTGAGAAACGTCTTATCAGTG
CCCGTCGAGCTGCTGTTGCGGCATCTGATGAGTTTGGCCTGCCGATTCTAGATCTAGACTCGTTTATGCTCGACGCCATT
CCTAAAGAGTTGATCGACGCCAAACTTATCGCTAAGCATCAGGTTATTCCGCTCATTAAACGCGAAAACCGTCTCTATGT
TGGCATCTCTGACCCAAGTAACATTCGCGCCCTCGATGAGCTTCGATTCAGTACTGGCATTCCAACTGAAGCCGTGATTG
TTGAAGAGGATAAACTGACTCGAAAAATCGAGCTGTTGATCGACTCTCAGGATGATGGCACAGCAGCGCTTGGAGACCTT
GCTGATAGCGATCTGGATAACCTCGAGCTGGGTGATACCGAGCGCCGCACTGAAGAGGATAGCGTTGCAGATGCTACCTC
TGATGCGCCAATCGTTCGATTTGTTAATAAGGTGCTACTCGATGCAATCAAGAATGGGGCATCCGATATTCACTTTGAAC
CCTATGAGAAGAGCTATCGCGTACGTTGTCGTATCGATGGCATTCTGCAGGAGGTGAGTAACCCTCCTATCAATCTTGCT
GCCCGCCTCTCTGCTCGTTTAAAGGTGATGTCGAACATGGACATTGCGGAGCGCCGTGTGCCGCAAGATGGACGTATCAA
ACTCAAAGTTTCCGCTAAACGCTCTATCGATTTTCGTGTGAATACACTTCCTACTCTATGGGGTGAGAAGATCGTACTTC
GTATTCTTGATCCAACCAGTGCCACCATCGGGGTTGATGCGCTTGGCTTTGAGCCTGAGCAGCGCGAACTCTTTATGAGT
GTACTGCGCCGCCCGCAGGGGATGGTGTTGGTTACGGGGCCTACCGGTTCAGGTAAAACGGTTACTCTCTATACTGGCCT
AAATATCCTCAACGAAGAGGAGCGCAACATCTCCACCGCTGAGGATCCAGTAGAGATTAACATGGAGGGGGTAAACCAGG
TCCATGTTAATCCAAAGGTTGGACTGACATTTGCTGAGGCCCTACGCGCCTTCTTGCGACAGGACCCTGACGTAGTCATG
GTCGGAGAGATTCGTGACTTAGAGACCGCCGAGATTGCCATTAAAGCAGCGCAGACGGGCCACATGGTGTTATCTACTCT
GCATACCAACAGCGCACCAGAGACACTGACGCGCCTTCGTAACATGGGTGTTGCCTCGTTTAACATCGCCACTTCCGTTA
CGCTGATCATTGCTCAGCGTCTGGCACGCCGTCTCTGTAGTAGCTGTAAAACTCAGGTTGACCTACCTCGCGCAACACTG
CTTGAAGAGGGTTTCACAGAGGAGCAGCTAGAGAAACTGACGATCTTTGAACACAACCCTGAGGGGTGTCCTCGATGTAA
CCGCGGTTACCGAGGACGTGTCGGTATTTATGAGATGTTGAAGGTATCGCCAGAGATAGCCGAGTGCATCATGAACAACG
GTAATGCTATCGAGATTTTAAAAGTCGCAAAATCTGAAGGCTTTGCTACTTTAAGAAGTGCAGGTCTTCGCAAGGTGGCA
GCAGGTGTCACAAGCCTTGAAGAGATGAATCGAGTTATTAAAACCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.609

100

0.586
  pilB Acinetobacter baylyi ADP1

58.711

99.826

0.586
  pilB Legionella pneumophila strain ERS1305867

52.348

100

0.523
  pilB Vibrio cholerae strain A1552

50.265

98.609

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

47.902

99.478

0.477
  pilB Vibrio campbellii strain DS40M4

47.972

98.609

0.473
  pilF Neisseria gonorrhoeae MS11

47.95

97.565

0.468
  pilF Thermus thermophilus HB27

37.871

99.652

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.871

99.652

0.377