Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | G8D25_RS09335 | Genome accession | NZ_CP051296 |
| Coordinates | 320170..320676 (-) | Length | 168 a.a. |
| NCBI ID | WP_014617872.1 | Uniprot ID | F6G3Z0 |
| Organism | Ralstonia solanacearum strain CIAT_078 | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
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Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Genomic island | 298432..321463 | 320170..320676 | within | 0 |
Gene organization within MGE regions
Location: 298432..321463
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G8D25_RS09240 (G8D25_09235) | - | 298432..299193 (+) | 762 | WP_168728909.1 | tyrosine-type recombinase/integrase | - |
| G8D25_RS09245 (G8D25_09240) | - | 299241..300451 (+) | 1211 | WP_086002887.1 | IS3 family transposase | - |
| G8D25_RS09250 (G8D25_09245) | - | 300810..301933 (+) | 1124 | WP_086002891.1 | IS3 family transposase | - |
| G8D25_RS09255 (G8D25_09250) | - | 302003..302581 (+) | 579 | WP_124994724.1 | hypothetical protein | - |
| G8D25_RS09260 (G8D25_09255) | - | 302744..306748 (+) | 4005 | Protein_306 | DEAD/DEAH box helicase | - |
| G8D25_RS09265 (G8D25_09260) | istA | 306778..307695 (+) | 918 | WP_061581096.1 | IS21 family transposase | - |
| G8D25_RS09270 (G8D25_09265) | istB | 307692..308478 (+) | 787 | Protein_308 | IS21-like element ISRso19 family helper ATPase IstB | - |
| G8D25_RS09275 (G8D25_09270) | - | 308560..308787 (+) | 228 | WP_247709234.1 | hypothetical protein | - |
| G8D25_RS09280 (G8D25_09275) | - | 309401..310603 (-) | 1203 | WP_014617863.1 | helix-turn-helix domain-containing protein | - |
| G8D25_RS09285 (G8D25_09280) | - | 310606..311268 (-) | 663 | WP_014617864.1 | hypothetical protein | - |
| G8D25_RS09290 (G8D25_09285) | - | 311522..312088 (+) | 567 | WP_042567706.1 | recombinase family protein | - |
| G8D25_RS09295 (G8D25_09290) | - | 312142..312906 (+) | 765 | WP_042567705.1 | hypothetical protein | - |
| G8D25_RS09300 (G8D25_09295) | - | 313018..313701 (+) | 684 | WP_124994723.1 | hypothetical protein | - |
| G8D25_RS09305 (G8D25_09300) | - | 313701..315125 (+) | 1425 | WP_168728911.1 | AAA family ATPase | - |
| G8D25_RS09310 (G8D25_09305) | - | 315091..316302 (-) | 1212 | WP_412771127.1 | IS3 family transposase | - |
| G8D25_RS09315 (G8D25_09310) | - | 316378..316644 (-) | 267 | WP_168728892.1 | hypothetical protein | - |
| G8D25_RS09320 (G8D25_09315) | istA | 316977..317993 (+) | 1017 | WP_039548595.1 | IS21 family transposase | - |
| G8D25_RS09325 (G8D25_09320) | istB | 317990..318304 (+) | 315 | Protein_319 | IS21-like element ISRso19 family helper ATPase IstB | - |
| G8D25_RS09330 (G8D25_09325) | - | 318349..320085 (-) | 1737 | WP_014617871.1 | PglL family O-oligosaccharyltransferase | - |
| G8D25_RS09335 (G8D25_09330) | pilA | 320170..320676 (-) | 507 | WP_014617872.1 | pilin | Machinery gene |
| G8D25_RS09340 (G8D25_09335) | pilA | 320942..321463 (-) | 522 | WP_014617873.1 | pilin | Machinery gene |
Sequence
Protein
Download Length: 168 a.a. Molecular weight: 16882.51 Da Isoelectric Point: 9.9749
>NTDB_id=437285 G8D25_RS09335 WP_014617872.1 320170..320676(-) (pilA) [Ralstonia solanacearum strain CIAT_078]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLAINATAAPAGQITITYTTAAGNGTLALVPTSGGNALVVSSAPGAAIMWTCYSAGKATAASSVAASPAATLLP
KYAPAECR
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLAINATAAPAGQITITYTTAAGNGTLALVPTSGGNALVVSSAPGAAIMWTCYSAGKATAASSVAASPAATLLP
KYAPAECR
Nucleotide
Download Length: 507 bp
>NTDB_id=437285 G8D25_RS09335 WP_014617872.1 320170..320676(-) (pilA) [Ralstonia solanacearum strain CIAT_078]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGCGACCTGTCTGTGGGCTCGTCGACGTTTGCT
GCTACCAAGAACGTGTCCGGCCTTGCTATTAACGCGACTGCGGCACCGGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTTGTCCCGACTTCGGGCGGTAATGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACCTGCTATTCTGCTGGTAAGGCCACGGCTGCCAGCAGCGTTGCTGCGAGCCCCGCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGCGACCTGTCTGTGGGCTCGTCGACGTTTGCT
GCTACCAAGAACGTGTCCGGCCTTGCTATTAACGCGACTGCGGCACCGGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTTGTCCCGACTTCGGGCGGTAATGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACCTGCTATTCTGCTGGTAAGGCCACGGCTGCCAGCAGCGTTGCTGCGAGCCCCGCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Ralstonia pseudosolanacearum GMI1000 |
79.167 |
100 |
0.792 |
| pilA2 | Legionella pneumophila str. Paris |
52.174 |
95.833 |
0.5 |
| pilA2 | Legionella pneumophila strain ERS1305867 |
52.174 |
95.833 |
0.5 |
| pilE | Neisseria elongata subsp. glycolytica ATCC 29315 |
38.265 |
100 |
0.446 |
| comP | Acinetobacter baylyi ADP1 |
42.353 |
100 |
0.429 |
| pilA/pilAI | Pseudomonas stutzeri DSM 10701 |
36.095 |
100 |
0.363 |