Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HEP75_RS05430 Genome accession   NZ_CP051261
Coordinates   1275896..1277635 (+) Length   579 a.a.
NCBI ID   WP_185825711.1    Uniprot ID   -
Organism   Xanthomonas sp. SI     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1269524..1285153 1275896..1277635 within 0


Gene organization within MGE regions


Location: 1269524..1285153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP75_RS05405 (HEP75_01059) - 1269524..1270684 (+) 1161 WP_185825706.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  HEP75_RS05410 (HEP75_01060) - 1270671..1271882 (-) 1212 WP_221899305.1 hypothetical protein -
  HEP75_RS05415 - 1271890..1272588 (-) 699 WP_185825708.1 class I SAM-dependent methyltransferase -
  HEP75_RS05420 (HEP75_01063) - 1273498..1274646 (-) 1149 WP_185825709.1 glycosyltransferase -
  HEP75_RS05425 (HEP75_01064) - 1274888..1275745 (-) 858 WP_185825710.1 glycosyltransferase family 2 protein -
  HEP75_RS05430 (HEP75_01065) pilB 1275896..1277635 (+) 1740 WP_185825711.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HEP75_RS05435 (HEP75_01066) pilR 1277729..1279117 (-) 1389 WP_185822434.1 sigma-54 dependent transcriptional regulator Regulator
  HEP75_RS05440 (HEP75_01067) - 1279191..1280804 (-) 1614 WP_185825712.1 HAMP domain-containing sensor histidine kinase -
  HEP75_RS05445 (HEP75_01068) sucC 1281104..1282267 (+) 1164 WP_179566646.1 ADP-forming succinate--CoA ligase subunit beta -
  HEP75_RS05450 (HEP75_01069) sucD 1282291..1283163 (+) 873 WP_179566644.1 succinate--CoA ligase subunit alpha -
  HEP75_RS05455 (HEP75_01070) - 1283246..1285153 (+) 1908 WP_185825713.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62871.18 Da        Isoelectric Point: 5.7922

>NTDB_id=437131 HEP75_RS05430 WP_185825711.1 1275896..1277635(+) (pilB) [Xanthomonas sp. SI]
MNAAVSANLVGITGIARRLVQDGALEEAVARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANAVEFGMPLLDVSVFDAS
QNAMKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGNSLG
GDDEGMDNLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRLAPAM
PENALLKEGFTEAEIAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIADAAQKIGVKDLRQSAL
IKARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=437131 HEP75_RS05430 WP_185825711.1 1275896..1277635(+) (pilB) [Xanthomonas sp. SI]
ATGAACGCAGCTGTCTCGGCCAACCTGGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGACGGCGCGCTGGAGGA
AGCGGTCGCGCGCACCGCCATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAATGGTTTTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCCGTCGAGTTCGGGATGCCGCTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAAAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTCGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGAGCAACGATGCGATCGGCAACAGCCTGGGC
GGCGACGACGAGGGTATGGATAACCTCGAAGTCGGTGCTGGCGACGAAGACATGGGCAGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCCTCGGATA
TCCATTTCGAGCCATACGAGGACGATTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAGCGTGGCCAAGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGTACGCTGCCGACCCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTATGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTATCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAAGATCCGGTCGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGGCTGGCCCCGGCCATG
CCGGAGAACGCGCTGTTGAAGGAAGGCTTCACCGAGGCCGAAATCGCCGCCGGCATCCAATTGTATGAGGCGGTGGGCTG
CGACGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTAATGCCGATGAACGACGAGATCGCGGCGATCG
TGCTGCAGGGCGGCAACGCGATGGACATCGCCGACGCGGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTG
ATCAAGGCGCGCGCCGGCATCACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.974

98.964

0.544
  pilB Acinetobacter baylyi ADP1

54.912

98.446

0.541
  pilB Legionella pneumophila strain ERS1305867

52.548

98.273

0.516
  pilF Neisseria gonorrhoeae MS11

48.944

98.1

0.48
  pilB Vibrio cholerae strain A1552

51.206

93.092

0.477
  pilB Vibrio campbellii strain DS40M4

46.14

98.446

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.018

97.582

0.449
  pilF Thermus thermophilus HB27

40.307

89.983

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.115

89.983

0.361