Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HEP73_RS05565 Genome accession   NZ_CP051189
Coordinates   1314624..1316363 (+) Length   579 a.a.
NCBI ID   WP_185822433.1    Uniprot ID   -
Organism   Xanthomonas sp. GW     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309624..1321363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP73_RS05545 (HEP73_01088) - 1310612..1311778 (-) 1167 WP_185822429.1 glycosyltransferase -
  HEP73_RS05550 (HEP73_01089) - 1311842..1312798 (-) 957 WP_185822430.1 lysylphosphatidylglycerol synthase domain-containing protein -
  HEP73_RS05555 (HEP73_01090) - 1312795..1313583 (-) 789 WP_185822431.1 class I SAM-dependent methyltransferase -
  HEP73_RS05560 (HEP73_01091) - 1313617..1314474 (-) 858 WP_185822432.1 glycosyltransferase family 2 protein -
  HEP73_RS05565 (HEP73_01092) pilB 1314624..1316363 (+) 1740 WP_185822433.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HEP73_RS05570 (HEP73_01093) pilR 1316519..1317907 (-) 1389 WP_185822434.1 sigma-54 dependent transcriptional regulator Regulator
  HEP73_RS05575 (HEP73_01094) - 1317982..1319595 (-) 1614 WP_185822435.1 HAMP domain-containing sensor histidine kinase -
  HEP73_RS05580 (HEP73_01095) sucC 1319895..1321058 (+) 1164 WP_179566646.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62901.20 Da        Isoelectric Point: 5.7937

>NTDB_id=436730 HEP73_RS05565 WP_185822433.1 1314624..1316363(+) (pilB) [Xanthomonas sp. GW]
MNAAVSANLVGITGIARRLVQDGALEETVARTAMAHAAEAKIPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSVFDAS
QNAMKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGNSLG
DDDEGMGNLEVGAGDEDMGGGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRLAPSM
PENALLKEGFTEAEIAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIADAAQKIGVKDLRQSAL
IKARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=436730 HEP73_RS05565 WP_185822433.1 1314624..1316363(+) (pilB) [Xanthomonas sp. GW]
ATGAACGCAGCTGTCTCGGCCAACCTGGTCGGCATCACCGGCATCGCACGCCGCCTGGTCCAGGACGGCGCACTGGAAGA
GACTGTCGCGCGGACCGCCATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTTTCCGAGAAGAAGCTGG
TCACCGCAGCGCAGCTGGCGGCCGCCAATGCGGTCGAGTTCGGGATGCCGCTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTGGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGAGCAACGATGCGATCGGCAACAGCTTGGGC
GACGATGATGAGGGCATGGGCAACCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCGGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAGTGTGGCCAAGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGTTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGGCTGGCCCCGTCCATG
CCGGAGAACGCGCTGTTGAAGGAAGGCTTTACCGAGGCCGAAATCGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTG
CGACGAATGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCCATTG
TGCTGCAGGGCGGCAACGCGATGGACATCGCCGATGCGGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTG
ATCAAGGCGCGCGCCGGCATCACCAGTCTGGCTGAGATCAATCGCGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.355

99.136

0.539
  pilB Acinetobacter baumannii D1279779

54.545

98.791

0.539
  pilB Legionella pneumophila strain ERS1305867

52.724

98.273

0.518
  pilB Vibrio cholerae strain A1552

49.654

99.827

0.496
  pilF Neisseria gonorrhoeae MS11

48.592

98.1

0.477
  pilB Vibrio campbellii strain DS40M4

46.14

98.446

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.702

96.891

0.453
  pilF Thermus thermophilus HB27

40.304

90.846

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.153

90.328

0.363