Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HF906_RS13700 Genome accession   NZ_CP051175
Coordinates   2933085..2933594 (-) Length   169 a.a.
NCBI ID   WP_043892119.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain Rs5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2928085..2938594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF906_RS13685 (HF906_13725) moaC 2929139..2929615 (+) 477 WP_003269419.1 cyclic pyranopterin monophosphate synthase MoaC -
  HF906_RS13690 (HF906_13730) - 2929729..2931179 (+) 1451 Protein_2687 IS1182 family transposase -
  HF906_RS13695 (HF906_13735) - 2931275..2933008 (-) 1734 WP_003269424.1 PglL family O-oligosaccharyltransferase -
  HF906_RS13700 (HF906_13740) pilA 2933085..2933594 (-) 510 WP_043892119.1 pilin Machinery gene
  HF906_RS13705 (HF906_13745) pilA 2933862..2934383 (-) 522 WP_043892120.1 pilin Machinery gene
  HF906_RS13710 (HF906_13750) - 2934567..2935265 (-) 699 WP_003269429.1 TerC family protein -
  HF906_RS13715 (HF906_13755) sucD 2935423..2936304 (-) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  HF906_RS13720 (HF906_13760) sucC 2936383..2937549 (-) 1167 WP_003261636.1 ADP-forming succinate--CoA ligase subunit beta -
  HF906_RS13725 (HF906_13765) - 2937589..2938245 (-) 657 WP_003269432.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17264.99 Da        Isoelectric Point: 9.9252

>NTDB_id=436607 HF906_RS13700 WP_043892119.1 2933085..2933594(-) (pilA) [Ralstonia solanacearum strain Rs5]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLGAGSSVFT
PTKNVADLKILGTDATAPGQITVTYTTAAGGGTLALVPSAAGNKLPLSSAPSGPIMWTCYAQNKAQAASSIAPSGTMTLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=436607 HF906_RS13700 WP_043892119.1 2933085..2933594(-) (pilA) [Ralstonia solanacearum strain Rs5]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGCTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCTATCGCCATTCCGGCATATCAGGACTACACCGTTCGTGCACGCGTGACGGAAGGCCTGTCGCTGG
CTGCGCAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGGGGGCGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCTGACCTCAAGATTCTCGGTACGGATGCTACTGCTCCGGGCCAGATCACCGTCACCTATACGAC
GGCAGCTGGCGGCGGCACGCTGGCTCTGGTTCCGTCTGCGGCAGGTAACAAGCTGCCGTTGAGCTCGGCACCGTCGGGCC
CGATCATGTGGACCTGCTACGCTCAAAACAAGGCTCAAGCAGCCAGCAGCATTGCTCCGAGCGGTACGATGACGCTGGCG
GCCAAGTACGCCCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(43-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

91.716

100

0.917
  pilA2 Legionella pneumophila str. Paris

51.235

95.858

0.491
  pilA2 Legionella pneumophila strain ERS1305867

50.617

95.858

0.485
  comP Acinetobacter baylyi ADP1

44.767

100

0.456
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.426
  pilA/pilA1 Eikenella corrodens VA1

41.722

89.349

0.373