Detailed information NTG0043654

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LP12_RS04675
• Genome accession: NC_016811
• Coordinates: 1021209..1022273 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila subsp. pneumophila ATCC 43290
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1016209..1027273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LP12_RS04665 (lp12_0947)	-	1017246..1019630 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
LP12_RS04670 (lp12_0948)	ravI	1019782..1020792 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
LP12_RS04675 (lp12_0949)	pilM	1021209..1022273 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
LP12_RS04680 (lp12_0950)	pilN	1022277..1022825 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
LP12_RS04685 (lp12_0951)	pilO	1022839..1023438 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
LP12_RS04690 (lp12_0952)	pilP	1023435..1024022 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
LP12_RS04695 (lp12_0953)	pilQ	1024026..1026122 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
LP12_RS04700 (lp12_0954)	aroK	1026568..1027095 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=43654 LP12_RS04675 WP_010946662.1 1021209..1022273(+) (pilM) [Legionella pneumophila subsp. pneumophila ATCC 43290]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=43654 LP12_RS04675 WP_010946662.1 1021209..1022273(+) (pilM) [Legionella pneumophila subsp. pneumophila ATCC 43290]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418