Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HF909_RS03175 Genome accession   NZ_CP051169
Coordinates   659675..661402 (-) Length   575 a.a.
NCBI ID   WP_193027556.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654675..666402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS03145 (HF909_03175) - 655298..655759 (+) 462 WP_193035777.1 NUDIX domain-containing protein -
  HF909_RS03150 (HF909_03180) yacG 655752..655967 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  HF909_RS03155 (HF909_03185) zapD 655978..656736 (-) 759 WP_020747465.1 cell division protein ZapD -
  HF909_RS03160 (HF909_03190) coaE 656854..657468 (-) 615 WP_193027553.1 dephospho-CoA kinase -
  HF909_RS03165 (HF909_03195) - 657481..658362 (-) 882 WP_282107753.1 prepilin peptidase -
  HF909_RS03170 (HF909_03200) pilC 658365..659630 (-) 1266 WP_193035781.1 type II secretion system F family protein Machinery gene
  HF909_RS03175 (HF909_03205) pilB 659675..661402 (-) 1728 WP_193027556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HF909_RS03180 (HF909_03210) - 661650..662927 (-) 1278 WP_193027557.1 HlyC/CorC family transporter -
  HF909_RS03190 (HF909_03220) ispB 663214..664182 (-) 969 WP_193028424.1 octaprenyl diphosphate synthase -
  HF909_RS03195 (HF909_03225) rplU 664462..664773 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  HF909_RS03200 (HF909_03230) rpmA 664811..665071 (+) 261 WP_020747472.1 50S ribosomal protein L27 -
  HF909_RS03205 (HF909_03235) obgE 665248..666348 (+) 1101 WP_020747473.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63717.50 Da        Isoelectric Point: 7.1546

>NTDB_id=436531 HF909_RS03175 WP_193027556.1 659675..661402(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2474]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIEAVIVEHDKLVRQLSLANEAPAEIKSLISKQETAQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQVLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKQGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=436531 HF909_RS03175 WP_193027556.1 659675..661402(-) (pilB) [Ralstonia pseudosolanacearum strain RUN2474]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGTGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCACAGTACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAAGCATTTC
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTGGCCACCTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGGCGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCTCCAAGCAGGAAACCGCGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGTACGCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGGTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCAGCGCTGAAGTCCTTCCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACTGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCAGGGCCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAGGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCAATCCAGGAGATCATCCTCAGCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCACTGCGCGATTCCGGCCTGCGGAAAGTCAAG
CAGGGGCTGACGTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.883

98.435

0.501
  pilF Neisseria gonorrhoeae MS11

52.963

93.913

0.497
  pilB Acinetobacter baylyi ADP1

49.374

97.217

0.48
  pilB Legionella pneumophila strain ERS1305867

47.695

98.087

0.468
  pilB Vibrio cholerae strain A1552

45.045

96.522

0.435
  pilB Vibrio campbellii strain DS40M4

45.203

94.261

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

44.954

94.783

0.426
  pilB/pilB1 Synechocystis sp. PCC 6803

35.574

100

0.377
  pilF Thermus thermophilus HB27

38.71

97.043

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.321

95.304

0.365