Detailed information NTG0436020

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HEQ83_RS12100
• Genome accession: NZ_CP051011
• Coordinates: 2513858..2514472 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain BY6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2508858..2519472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HEQ83_RS12070	rpsT	2509158..2509424 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HEQ83_RS12075	holA	2509441..2510482 (-)	1042	Protein_2341	DNA polymerase III subunit delta	-
HEQ83_RS12080	-	2510522..2510676 (-)	155	Protein_2342	hypothetical protein	-
HEQ83_RS12085	-	2510717..2510851 (+)	135	WP_003152870.1	YqzM family protein	-
HEQ83_RS12090	comEC	2510870..2513221 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HEQ83_RS12095	-	2513222..2513791 (-)	570	WP_003152868.1	ComE operon protein 2	-
HEQ83_RS12100	comEA	2513858..2514472 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
HEQ83_RS12105	comER	2514531..2515352 (+)	822	WP_014305449.1	late competence protein ComER	-
HEQ83_RS12110	-	2515421..2516158 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
HEQ83_RS12115	rsfS	2516155..2516511 (-)	357	WP_003152864.1	ribosome silencing factor	-
HEQ83_RS12120	yqeK	2516508..2517089 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HEQ83_RS12125	-	2517079..2517648 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
HEQ83_RS12130	yhbY	2517659..2517949 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HEQ83_RS12135	aroE	2517943..2518785 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=436020 HEQ83_RS12100 WP_003152867.1 2513858..2514472(-) (comEA) [Bacillus velezensis strain BY6]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=436020 HEQ83_RS12100 WP_003152867.1 2513858..2514472(-) (comEA) [Bacillus velezensis strain BY6]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363