Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FOB43_RS13240 Genome accession   NZ_CP050992
Coordinates   2781563..2783269 (+) Length   568 a.a.
NCBI ID   WP_168227890.1    Uniprot ID   -
Organism   Chromobacterium violaceum strain FDAARGOS_635     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2776563..2788269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB43_RS13220 (FOB43_13220) ffh 2777205..2778560 (-) 1356 WP_011137380.1 signal recognition particle protein -
  FOB43_RS13225 (FOB43_13225) ccsA 2778674..2779495 (+) 822 WP_011137379.1 cytochrome c biogenesis protein CcsA -
  FOB43_RS13230 (FOB43_13230) - 2779564..2779881 (+) 318 WP_011137378.1 helix-hairpin-helix domain-containing protein -
  FOB43_RS13235 (FOB43_13235) - 2780288..2781394 (+) 1107 WP_168227889.1 porin -
  FOB43_RS13240 (FOB43_13240) pilF 2781563..2783269 (+) 1707 WP_168227890.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB43_RS13245 (FOB43_13245) pilC 2783395..2784618 (+) 1224 WP_011137374.1 type II secretion system F family protein Machinery gene
  FOB43_RS13250 (FOB43_13250) - 2784630..2785535 (+) 906 WP_168227891.1 A24 family peptidase -
  FOB43_RS13255 (FOB43_13255) coaE 2785520..2786134 (+) 615 WP_011137372.1 dephospho-CoA kinase -
  FOB43_RS13260 (FOB43_13260) zapD 2786171..2786929 (+) 759 WP_031297029.1 cell division protein ZapD -
  FOB43_RS13265 (FOB43_13265) yacG 2786935..2787126 (+) 192 WP_168227892.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62213.74 Da        Isoelectric Point: 5.8806

>NTDB_id=435801 FOB43_RS13240 WP_168227890.1 2781563..2783269(+) (pilF) [Chromobacterium violaceum strain FDAARGOS_635]
MEATAGISGLGRALVQHNMLLQQDADAISKQAVTSHLSFVEQLIQSKKMAARDIAMFSSRVFGYPLLDLASLDTDQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPDAQLDSGPQTEVDDAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQLGFEPEQKAHLLKAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEGMVDLRRAGLLKVMSGVTSLA
EVEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=435801 FOB43_RS13240 WP_168227890.1 2781563..2783269(+) (pilF) [Chromobacterium violaceum strain FDAARGOS_635]
ATGGAAGCTACCGCAGGGATTTCCGGCCTGGGCAGGGCCCTGGTCCAGCACAATATGCTGTTGCAGCAGGATGCGGACGC
CATTTCGAAACAGGCGGTCACTTCCCATCTCAGTTTTGTCGAGCAGCTGATCCAGAGCAAGAAGATGGCCGCGCGCGACA
TCGCGATGTTTTCATCGCGGGTGTTCGGCTACCCGCTGCTGGATCTGGCTTCGCTGGATACCGACCAGCTACCCAAAGGG
CTGGTGGATGACGAAGTGATCGCCAGCCGCCGCCTTTTGCCGTTGCTGAAGCGCGGCAACAAGCTGTATATCGGCACTTC
CGATCCTACCCAGACCTCGGCCTATCATGCGATCACCTTCAAGACCGGCCTGACGGTCGAGGTGGTGGTGGTGGAAGAGG
ACAAGCTGTCCAAGCTGATCGGCCGTTACCAGGACAATTCGACCTCGGCTTTGAAGGAGCTGGAGAGCGAGGACTTCGGC
GACCTGGAGTTCGCCGATCCGGACGCCCAGCTCGACAGCGGCCCGCAGACCGAGGTCGACGACGCGCCGGTGGTCAAGTT
CATCCACAAGATGCTGCTGGACGCGATAGGCATCGGCGCGTCCGACATCCACTTCGAACCATACGAAAAATATTACCGCA
TCCGCTACCGGGTGGACGGCGTGCTGAAGGAGGTGGCGCAGCCGCCCTTGCTGCTGAAGGAAAAGATCGCCTCCCGCATC
AAGGTGATTTCCAGGCTGGACATTTCGGAAAAGCGCTTGCCGCAGGATGGCCGGTTGAAGTTGGTGATTTCCAAGACCCG
GGCGATAGATTTCCGCGTCAGCACGCTGCCCACGCTGTACGGCGAGAAGATCGTCATGCGTATCCTGGACTCGTCCGGCG
CCTCGCTGGATATCGACCAGCTGGGTTTCGAGCCGGAGCAGAAGGCGCATCTGCTGAAGGCGATCGCCAGGCCGTACGGC
ATGGTGCTGGTGACCGGTCCGACGGGCAGCGGCAAGACAGTGTCGCTGTACACCTGTCTGAACATCCTGAACAAGCCGGA
CACCAATATCTCCACGGCGGAGGATCCGGTTGAAATCAACCTTCCCGGCATCAACCAGGTCAACGTCAACGAGAAGGCCG
GGCTGACCTTCGCGTCGGCGCTGAAAGCCTTCTTGCGGCAGGATCCGGACGTGATCATGGTCGGCGAAATCCGCGATTTC
GAAACCGCCGACATCGCCATCAAGGCGGCTCAGACCGGCCACATGGTGTTTTCCACGCTGCATACCAATGACGCCCCGTC
CACGCTGACCCGGATGCTGAATATGGGCGTGGCGCCGTTCAACATCGCCAGCTCGGTGTTGCTGATCATGGCGCAGCGCC
TGGCGCGCCGCTTGTGCAGCCATTGCAAGCAGCCGGTGGAAATCCCGAAGGAAGCGCTGCTGCGCGCGGGTTTTACCAAA
GAGGACCTGGCGGGCGGCTGGCATCCGTACGGGCCGGTTGGCTGCGAGGAGTGCCGGAATACCGGCTACAAAGGCCGGGT
CGGCATTTTCGAAGCCATGCCGATCAGCGACGCGATGATGCGCTTGATCATGAAGAACGGCACCTCGGTCGACATCTCCG
ATCTGGCCAAGGAGGAGGGCATGGTCGATCTGCGCCGCGCCGGCCTGTTGAAGGTGATGAGCGGAGTGACTTCGCTGGCC
GAGGTCGAAGCGGTAACCAACGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

58.541

98.944

0.579
  pilB Acinetobacter baumannii D1279779

55.308

100

0.569
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.229

100

0.537
  pilB Vibrio cholerae strain A1552

49.732

98.415

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.043

98.944

0.475
  pilB Vibrio campbellii strain DS40M4

46.181

99.12

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.036

97.183

0.389
  pilF Thermus thermophilus HB27

38.612

98.944

0.382